Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis

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	Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis
	Zhang, GT; czhang2006@gmail.com; Zhang, CS; Ju, JH; Zhang, S; Tian, XP; Zhang, HB; Zhu, YG; Li, SM; Zhang, QB
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2012
卷号	134 期号:21 页码:8996-9005
ISSN号	0002-7863
英文摘要	Xiamycin A (XMA) and oxiamycin (OXM) are bacterial indolosesquiterpenes featuring rare pentacyclic ring systems and are isolated from a marine-derived Streptomyces sp. SCSIO 02999. The putative biosynthetic gene cluster for XMA/OXM was identified by a partial genome sequencing approach. Eighteen genes were proposed to be involved in XMA/OXM biosynthesis, including five genes for terpene synthesis via a non-mevalonate pathway, eight genes encoding oxidoreductases, and five genes for regulation and resistance. Targeted disruptions of 13 genes within the xia gene cluster were carried out to probe their encoded functions in XMA/OXM biosynthesis. The disruption of xiaK, encoding an aromatic ring hydroxylase, led to a mutant producing indosespene and a minor amount of XMA. Feeding of indosespene to XMA/OXM nonproducing mutants revealed indosespene as a common precursor for XMA/OXM biosynthesis. Most notably, the flavin dependent oxygenase XiaI was biochemically characterized in vitro to convert indosespene to XMA, revealing an unusual oxidative cyclization strategy tailoring indolosesquiterpene biosynthesis.
学科主题	Chemistry, Multidisciplinary
资助项目	LMB
语种	英语
WOS记录号	WOS:000304570700044
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9898]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	czhang2006@gmail.com
推荐引用方式 GB/T 7714	Zhang, GT,czhang2006@gmail.com,Zhang, CS,et al. Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(21):8996-9005.
APA	Zhang, GT.,czhang2006@gmail.com.,Zhang, CS.,Ju, JH.,Zhang, S.,...&Li, HX.(2012).Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,134(21),8996-9005.
MLA	Zhang, GT,et al."Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134.21(2012):8996-9005.