Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization
	He, XC; Wang, YH; Fu, DK; Luo, P; yhwang@scsio.ac.cn
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2012
卷号	7 期号:3 页码:243-247
关键词	Immune-relevant Gene Pinctada Martensii Suppression Subtractive Hybridization (Ssh) Vibrio Alginolyticus
ISSN号	1744-117X
英文摘要	One hemolymph subtracted cDNA library of pearl oyster (Pinctada martensii, Dunker 1837) was constructed using the suppression subtractive hybridization (SSH) in response to Vibrio alginolyticus. A total of 1089 clones were sequenced. All the consensuses were recognized based on the BLAST searches in NCBI, and revealed that 376 (58%) of them had no significant matches to reported sequences in the database. 267 ESTs were in significant matches after homologous sequence searches. Hypothesized genes inferred from EST sequences were categorized into six groups according to their putative biological functions: replication, transcription and translation: cellular processes; responded to stimuli; metabolism and biosynthesis: signal transduction genes; "other" category. The five genes, pearlin gene promoter PGPPm, serine/threonine kinase STKPm, limbic system-associated membrane protein LSAMPPm, nacrein gene intron 6 NGIPm6 and ferritin-like protein FLPPm, were analyzed using real-time PCR. All these genes were significantly expressed after V. alginolyticus challenge. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
资助项目	LMB
语种	英语
WOS记录号	WOS:000308115900002
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9806]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	yhwang@scsio.ac.cn
推荐引用方式 GB/T 7714	He, XC,Wang, YH,Fu, DK,et al. Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2012,7(3):243-247.
APA	He, XC,Wang, YH,Fu, DK,Luo, P,&yhwang@scsio.ac.cn.(2012).Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,7(3),243-247.
MLA	He, XC,et al."Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 7.3(2012):243-247.