Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods
	Chen, HH; Qin, S; Zhao, GZ; Li, J; Zhu, WY; Xu, LH; Jiang, JH; Li, WJ; wjli@ynu.edu.cn
刊名	ENVIRONMENTAL MICROBIOLOGY REPORTS
	2012
卷号	4 期号:5 页码:522-531
ISSN号	1758-2229
英文摘要	Endophytes are now considered as an important component of biodiversity. However, the diversity of endophytic actinobacteria associated with tropical rainforest native medicinal plants is essentially unknown. In this study, the diversity of endophytic actinobacteria residing in root, stem and leaf tissues of medicinal plant Maytenus austroyunnanensis collected from tropical rainforest in Xishuangbanna, China was investigated with a combination of cultivation and culture-independent analysis on the basis of 16S rRNA gene sequencing. By using different selective isolation media and methods, a total of 312 actinobacteria were obtained, and they were affiliated with the order Actinomycetales (distributed into 21 genera). Based on a protocol for endophytes enrichment, three 16S rRNA gene clone libraries were constructed and 84 distinct operational taxonomic units were identified and they distributed among the orders Actinomycetales and Acidimicrobiales, including eight suborders and at least 38 genera with a number of rare actinobacteria genera. Phylogenetic analysis showed that 32% of the clones in the libraries had lower than 97% similarities with related type strains. Interestingly, six genera from the order Actinomycetales and uncultured clones from Acidimicrobiales have not, to our knowledge, been previously reported as endophytes. Our study confirms abundant endophytic actinobacterial consortium in tropical rainforest native plant and suggests that this special habitat represents an underexplored reservoir of diverse and novel actinobacteria of potential interest for bioactive compounds discovery.
学科主题	Environmental Sciences ; Microbiology
资助项目	LMB
语种	英语
WOS记录号	WOS:000309445800008
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9786]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	wjli@ynu.edu.cn
推荐引用方式 GB/T 7714	Chen, HH,Qin, S,Zhao, GZ,et al. Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods[J]. ENVIRONMENTAL MICROBIOLOGY REPORTS,2012,4(5):522-531.
APA	Chen, HH.,Qin, S.,Zhao, GZ.,Li, J.,Zhu, WY.,...&wjli@ynu.edu.cn.(2012).Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods.ENVIRONMENTAL MICROBIOLOGY REPORTS,4(5),522-531.
MLA	Chen, HH,et al."Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods".ENVIRONMENTAL MICROBIOLOGY REPORTS 4.5(2012):522-531.