The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk
	Ye, Y; Wu, Q; Xu, X; Yang, X; Dong, X; Zhang, J; yeyw04@mails.gucas.ac.cn; wuqp203@yahoo.com.cn
刊名	JOURNAL OF DAIRY SCIENCE
	2010
卷号	93 期号:6 页码:2315-2320
关键词	Enterobacter Sakazakii Enterobacterial Repetitive Intergenic Consensus (Eric)-pcr Random Amplified Polymorphic Dna (Rapd)-pcr Antibiotic Resistance Test
ISSN号	0022-0302
英文摘要	Enterobacter sakazakii is an emerging foodborne pathogen associated with severe diseases in neonates. Infant formula milk (IFM) has been identified as one of the major contaminated sources and a transmission vehicle. To determine the phenotypic and genotypic characterization of this pathogen, 22 E. sakazakii strains isolated from IFM by an FDA-recommended method and PCR on the a-glucosidase gene were subtyped by random amplified polymorphic DNA (RAPD)-PCR, enterobacterial repetitive intergenic consensus (ERIC)-PCR, and antibiotic resistance patterns. At a similarity threshold of 80%, 16 ERIC-PCR fingerprint types were identified with a discriminatory power (D) of 0.933, and 18 RAPD-PCR types were identified with D of 0.973. Resistance to 9 antibiotics tested by disk diffusion assay revealed 6 antibiotic resistance patterns with D of 0.749. The comparison of characterization indicated that RAPD-PCR and ERIC-PCR have high discriminatory power showing genetic diversity of E. sakazakii isolates, and ERIC-PCR patterns showed a closer correlation than RAPD-PCR patterns to phenotypic characterization and the brands of IFM. Overall, the ERIC-PCR typing method could be used for tracing sources of E. sakazakii isolates in the food chain.
学科主题	Agriculture, Dairy & Animal Science ; Food Science & Technology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000277929100002
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3836]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	yeyw04@mails.gucas.ac.cn; wuqp203@yahoo.com.cn
推荐引用方式 GB/T 7714	Ye, Y,Wu, Q,Xu, X,et al. The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk[J]. JOURNAL OF DAIRY SCIENCE,2010,93(6):2315-2320.
APA	Ye, Y.,Wu, Q.,Xu, X.,Yang, X.,Dong, X.,...&wuqp203@yahoo.com.cn.(2010).The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk.JOURNAL OF DAIRY SCIENCE,93(6),2315-2320.
MLA	Ye, Y,et al."The phenotypic and genotypic characterization of Enterobacter sakazakii strains from infant formula milk".JOURNAL OF DAIRY SCIENCE 93.6(2010):2315-2320.