Sex-biased regulation of respiratory burst, phagocytic activity and plasma immune factors in lined seahorse (Hippocampus erectus) after subchronic benzo[a]pyrene exposure

doi:10.1016/j.fsi.2018.12.068

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Sex-biased regulation of respiratory burst, phagocytic activity and plasma immune factors in lined seahorse (Hippocampus erectus) after subchronic benzo[a]pyrene exposure
	Ren, Chuanbo 3; Jiang, Han ; Wang, Kai ; Fang, Yan ; Chen, Jun ; Li, Yanmin 2; Xia, Guangbo ; Zhang, Yingfeng ; Liu, Yu ; Lin, Qiang 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019
卷号	86 页码:1162
关键词	Hippocampus erectus Immunocompetence Marine pollution benzo[a]pyrene Sexual dimorphism
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.12.068
英文摘要	Both wild and aquacultured seahorses are currently under great threat from marine pollution, notably from the potent contaminant and carcinogen benzo[a]pyrene (BaP). However, very little data are available regarding the immunomodulating effects of BaP in seahorses. Therefore, in this study, we exposed lined seahorses (Hippocampus erectus) for 7 d to BaP at three dosages (0.5, 5, and 50 mu g/L) to evaluate sexual dimorphism in immune response. We measured eight immune parameters in the blood, including respiratory burst (RB), phagocytic activity (PA), monocytes/leucocytes, immunoglobulin M, complement 3, complement, interferon-a, and interleukin-2. Male seahorses had significantly higher parameters than females, except in terms of monocytes/leucocytes (P < 0.05). Although flow cytometry showed that RB and PA variation per BaP dose were roughly similar across sexes, RB and PA exhibited distinct patterns. Additionally, fluorescence intensity and leucocyte percentage were positively correlated in PA but not RB for all treatment and sex combinations. Through ELISA, we showed that the other six parameters had complex responses that nevertheless varied in a BaP-dosage and sex-dependent manner. Overall, adult male seahorses had higher immunocompetence than females before BaP exposure, and sexual dimorphism continued to be apparent during BaP exposure. Furthermore, all eight parameters were sensitive to BaP. Based on these results, we highly recommend H. erectus as a sentinel species for crude contamination, whereas PA and RB are valuable bioindicators of marine contaminants such as BaP.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17888]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Shandong Marine Resource & Environm Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai, Peoples R China 2.Ludong Univ, Sch Agr, 186 Hongqing Middle Rd, Yantai 264025, Peoples R China 3.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Ren, Chuanbo,Jiang, Han,Wang, Kai,et al. Sex-biased regulation of respiratory burst, phagocytic activity and plasma immune factors in lined seahorse (Hippocampus erectus) after subchronic benzo[a]pyrene exposure[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1162, 1168.
APA	Ren, Chuanbo.,Jiang, Han.,Wang, Kai.,Fang, Yan.,Chen, Jun.,...&Lin, Qiang.(2019).Sex-biased regulation of respiratory burst, phagocytic activity and plasma immune factors in lined seahorse (Hippocampus erectus) after subchronic benzo[a]pyrene exposure.FISH & SHELLFISH IMMUNOLOGY,86,1162.
MLA	Ren, Chuanbo,et al."Sex-biased regulation of respiratory burst, phagocytic activity and plasma immune factors in lined seahorse (Hippocampus erectus) after subchronic benzo[a]pyrene exposure".FISH & SHELLFISH IMMUNOLOGY 86(2019):1162.