Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746

doi:10.1007/s00253-019-09759-2

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746
	Hu, Jiangchun ; Shao, Mingwei 1; Pan, Huaqi 1; Ju, Jianhua 1; Tian, Xinpeng 1; Huang, Hongbo 1; Xie, Yunchang 1
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2019
卷号	103 期号:10 页码:4153
关键词	Bacillus Genome Secondary metabolite Siderophore Non-ribosomal peptide
ISSN号	0175-7598
DOI	10.1007/s00253-019-09759-2
英文摘要	Bacillus spp. are important producers of bioactive natural products with potential applications in medicine and agriculture. Bacillus sp. SCSIO 05476 from a deep-sea sediment exhibits broad-spectrum antimicrobial activities and strong cytotoxic activity. Here, an integrative approach combining genome mining and metabolic profiling has been applied to decipher the chemical origins of this strain's varied and significant biological activities. First, genome mining revealed 19 candidate gene clusters encoding the biosynthesis of diverse secondary metabolites. Then, a series of bacillibactins, fengycins, bacillomycins, surfactins, bacillaenes, macrolactins, and related species were found by LC-DAD-MS. Finally, three new linear bacillibactins, linbacillibactins A-C (1-3), along with 11 known secondary metabolites, bacillibactin (4), normal-C-13 Val(7) surfactin (5), anteiso-C-13 Leu(7) surfactin (6), iso-C-14 Leu(7) surfactin (7), normal-C-14 Leu(7) surfactin (8), anteiso-C-14 Leu(7) surfactin (9), macrolactin D (10), normal-C-14 bacillomycin D (11), iso-C-16 bacillomycin D (12), normal-C-17 bacillomycin D (13), and iso-C-17 bacillomycin D (14), were obtained and elucidated by bioactivity and structure-guided isolation from the fermentation of strain SCSIO 05746. Among them, new compounds 1-3 show significant siderophore activities comparable to that of bacillibactin (4), compounds 13 and 14 exhibit strong cytotoxic activity. At the same time, the strain classification status was confirmed by genomic analyses, and the complete genome sequence of Bacillus siamensis was presented firstly. This study provides a foundation for understanding the mechanisms driving SCSIO 05746's multiple bioactivities and demonstrates a successful way of discovering bioactive metabolites using a combination of genome mining and metabolic profiling methods.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17856]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Hu, Jiangchun,Shao, Mingwei,Pan, Huaqi,et al. Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(10):4153, 4165.
APA	Hu, Jiangchun.,Shao, Mingwei.,Pan, Huaqi.,Ju, Jianhua.,Tian, Xinpeng.,...&Xie, Yunchang.(2019).Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(10),4153.
MLA	Hu, Jiangchun,et al."Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.10(2019):4153.