Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate

doi:10.1080/10242422.2018.1526926

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate
	Zhang, Yun 1; Sai, Ke 3,4,5; Hu, Yunfeng 1; Xu, Shan 2; Li, Renqiang 2; Sun, Aijun 1; Wang, Yilong 1
刊名	BIOCATALYSIS AND BIOTRANSFORMATION
	2019
卷号	37 期号:3 页码:190
关键词	Biocatalysis novel esterase kinetic resolution L-methyl lactate opposite enantioselectivity
ISSN号	1024-2422
DOI	10.1080/10242422.2018.1526926
英文摘要	Chiral lactic acids and their ester derivatives are crucial building blocks and intermediates for the synthesis of a great variety of valuable functional materials and pharmaceuticals. Before our study, the reports about the enantioselective preparation of pure L-lactic acid and its ester derivatives through direct hydrolysis of racemic substrate were quite rare. Herein, we heterologously expressed and functionally characterized one novel microbial esterase WDEst9 from Dactylosporangium aurantiacum, which exhibited high resistance to diverse metal ions, organic solvents, surfactants, NaCl and KCl. We further utilized WDEst9 as a green biocatalyst in the kinetic resolution of (+/-)-methyl lactate through direct hydrolysis and generated L-methyl lactate with high enantiomeric excess (e.e. >99%) and high yield (>86%) after process optimization. Notably, the enantioselectivity of WDEst9 was opposite than that of two previously reported esterases PHE14 and BSE01701 that can generate D-methyl lactate though kinetic resolution of (+/-)-methyl lactate. Microbial esterase WDEst9 is a promising green biocatalyst in the preparation of valuable chiral chemicals and opens the door for the identification of useful industrial enzymes and biocatalysts from the genus Dactylosporangium.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17848]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Materia Med, Guangzhou, Guangdong, Peoples R China 3.Jinan Univ, Dept Biotechnol, Guangzhou, Guangdong, Peoples R China 4.Sun Yat Sen Univ, Canc Ctr, Dept Neurosurg Neurooncol, Guangzhou, Guangdong, Peoples R China 5.Collaborat Innovat Ctr Canc Med, Guangzhou, Guangdong, Peoples R China 6.State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yun,Sai, Ke,Hu, Yunfeng,et al. Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2019,37(3):190, 200.
APA	Zhang, Yun.,Sai, Ke.,Hu, Yunfeng.,Xu, Shan.,Li, Renqiang.,...&Wang, Yilong.(2019).Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate.BIOCATALYSIS AND BIOTRANSFORMATION,37(3),190.
MLA	Zhang, Yun,et al."Characterization of one novel microbial esterase WDEst9 and its use to make l-methyl lactate".BIOCATALYSIS AND BIOTRANSFORMATION 37.3(2019):190.