Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata

doi:10.1007/s00343-019-8154-5

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata
	Liu Xiande 2; Wu Xiangwei 1,3; Yu Ziniu 3
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2019
卷号	37 期号:4 页码:1301
关键词	Paphia undulata foot tissue transcriptome innate immunity unigene microsatellite
ISSN号	2096-5508
DOI	10.1007/s00343-019-8154-5
英文摘要	Increasingly, exogenous stressors such as pathogen infections, variable water conditions, and pollution are resulting in high mortality of Paphia undulata, deleteriously affecting the quality of clam harvests. The foot is a burrowing organ in clams. Physical damage and constant contact with the external environment cause the foot to be highly sensitive to pathogen invasion and water condition variation. In the present study, the foot tissue transcriptome was analyzed to identify genes involved in immune and stress responses. The P. undulata transcriptome included 5 286 668 078 bp reads generated by Illumina Hiseq 2000 sequencing and were assembled into 1 785 226 contigs by de novo method. The contigs were clustered into 99 339 transcripts and further grouped into 60 201 unigenes. Of them, 22 260 unigenes were successfully annotated using public databases. Twelve genes that were response to immune and stress were identified with abundant expression levels, including heat shock protein 70, cold shock protein, complement C3, cathepsin L, ubiquitin carboxyl-terminal hydrolase L5, and translationally controlled tumor protein. Furthermore, 566 unigenes were found homologous to genes involved in the immune response systems of pathogen discrimination, signal transduction, and immune effector, such as lectins, toll-like receptors, complement pathway, toll-like receptor signaling pathway, heat shock proteins, antioxidant enzymes, lysozymes, and mucins, indicating that P. undulata could have a complete set of innate immune mechanisms. In addition, 4 270 microsatellite markers (SSRs) were identified from 60 201 unigenes, of which trinucleotide repeats were most abundant and 16 SSRs were tested to be polymorphic. The present study provides a new insight into innate immunity and stress response mechanisms in P. undulata.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17816]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China 2.Yunnan Agr Univ, Anim Sci & Technol Coll, Kunming 650201, Yunnan, Peoples R China 3.Jimei Univ, Minist Agr China, Key Lab Hlth Mariculture East China Sea, Fisheries Coll, Xiamen 361021, Fujian, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu Xiande,Wu Xiangwei,Yu Ziniu. Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(4):1301, 1316.
APA	Liu Xiande,Wu Xiangwei,&Yu Ziniu.(2019).Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(4),1301.
MLA	Liu Xiande,et al."Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.4(2019):1301.