Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa

doi:10.1111/1462-2920.14595

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	Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa
	Wang, Xiaoxue 1; Ni, Songwei ; Tang, Kaihao ; Li, Yangmei 1; Sun, Chenglong 1,2; Guo, Yunxue
刊名	ENVIRONMENTAL MICROBIOLOGY
	2019
卷号	21 期号:8 页码:2707
ISSN号	1462-2912
DOI	10.1111/1462-2920.14595
英文摘要	Toxin/antitoxin (TA) systems are ubiquitous in bacteria and archaea and participate in biofilm formation and stress responses. The higBA locus of the opportunistic pathogen Pseudomonas aeruginosa encodes a type II TA system. Previous work found that the higBA operon is cotranscribed and that HigB toxin regulates biofilm formation and virulence expression. In this study, we demonstrate that HigA antitoxin is produced at a higher level than HigB and that higA mRNA is expressed separately from a promoter inside higB during the late stationary phase. Critically, HigA represses the expression of mvfR, which is an important virulence-related regulator, by binding to a conserved HigA palindrome (5 '-TTAAC GTTAA-3 ') in the mvfR promoter, and the binding of HigB to HigA derepresses this process. During the late stationary phase, excess HigA represses the expression of mvfR and higBA. However, in the presence of aminoglycoside antibiotics where Lon protease is activated, the degradation of HigA by Lon increases P. aeruginosa virulence by simultaneously derepressing mvfR and higB transcription. Therefore, this study reveals that the antitoxin of the P. aeruginosa TA system is integrated into the key virulence regulatory network of the host and functions as a transcriptional repressor to control the production of virulence factors.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17802]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Guizhou Univ Tradit Chinese Med, Basic Med Sch, Guiyang, Guizhou, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaoxue,Ni, Songwei,Tang, Kaihao,et al. Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa[J]. ENVIRONMENTAL MICROBIOLOGY,2019,21(8):2707, 2723.
APA	Wang, Xiaoxue,Ni, Songwei,Tang, Kaihao,Li, Yangmei,Sun, Chenglong,&Guo, Yunxue.(2019).Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa.ENVIRONMENTAL MICROBIOLOGY,21(8),2707.
MLA	Wang, Xiaoxue,et al."Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa".ENVIRONMENTAL MICROBIOLOGY 21.8(2019):2707.