Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis

doi:10.1016/j.biortech.2019.121720

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	Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis
	Wang, Xiang 1; Li, Hong-Ye ; Zhang, Lin 3; Liu, Jie-Sheng ; Yang, Wei-Dong ; Balamurugan, Srinivasan ; Liu, Yu-Hong ; Alimujiang, Adili ; Luo, Shan-Wei
刊名	BIORESOURCE TECHNOLOGY
	2019
卷号	289 页码:121720
关键词	Haematococcus pluvialis Astaxanthin Ethanol induction Jasmonate pathway Transcriptional regulation
ISSN号	0960-8524
DOI	10.1016/j.biortech.2019.121720
英文摘要	Haematococcus pluvialis is a main biological resource for the antioxidant astaxanthin production, however, potential modulators and molecular mechanisms underpinning astaxanthin accumulation remain largely obscured. We discovered that provision of ethanol (0.4%) significantly triggered the cellular astaxanthin content up to 3.85% on the 4th day of treatment. Amongst, 95% of the accumulated astaxanthin was esterified, particularly enriched with monoesters. Ultrastructural analysis revealed that ethanol altered cell wall structure and physiological properties. Antioxidant analyses revealed that astaxanthin accumulation offset the ethanol induced oxidative stress. Ethanol treatment reduced carbohydrates while increased lipids and jasmonic acid production. Transcriptomic analysis uncovered that ethanol orchestrated the expression of crucial genes involved in carotenogenesis, e.g. PSY, BKT and CRTR-b were significantly upregulated. Moreover, methyl jasmonic acid synthesis was induced and played a major role in regulating the carotenogenic genes. The findings uncovered the novel viewpoint in the intricate transcriptional regulatory mechanisms of astaxanthin biosynthesis.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17798]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Georgetown Univ, Dept Biostat Bioinformat & Biomath, Washington, DC 20057 USA 2.Jinan Univ, Coll Life Sci & Technol, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Guangzhou 510632, Guangdong, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 4.City Univ Hong Kong Kowloon, Sch Energy & Environm, Kowloon, Hong Kong 999077, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiang,Li, Hong-Ye,Zhang, Lin,et al. Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis[J]. BIORESOURCE TECHNOLOGY,2019,289:121720.
APA	Wang, Xiang.,Li, Hong-Ye.,Zhang, Lin.,Liu, Jie-Sheng.,Yang, Wei-Dong.,...&Luo, Shan-Wei.(2019).Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis.BIORESOURCE TECHNOLOGY,289,121720.
MLA	Wang, Xiang,et al."Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis".BIORESOURCE TECHNOLOGY 289(2019):121720.