Utilization of one novel deep-sea microbial protease sin3406-1 in the preparation of ethyl (S)-3-hydroxybutyrate through kinetic resolution

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Utilization of one novel deep-sea microbial protease sin3406-1 in the preparation of ethyl (S)-3-hydroxybutyrate through kinetic resolution
	Hu, Yunfeng 1,2; Huang, Jinlong 2,3; Xu, Yongkai 4; Zhang, Yun 2; Sun, Aijun 2
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2018
卷号	34 期号:9 页码:124
关键词	Biocatalysis Marine microorganisms Novel protease Kinetic resolution Ethyl (S)-3-hydroxybutyrate
ISSN号	0959-3993
英文摘要	One novel protease sin3406-1 was identified from Streptomyces niveus SCSIO 3406, which was isolated from the deep sea of the South China Sea, and heterologously expressed in E. coli BL21(DE3). Protease sin3406-1 was further used as a green biocatalyst in the kinetic resolution of racemic ethyl-3-hydroxybutyrate. After careful process optimization, chiral product ethyl (S)-3-hydroxybutyrate was generated with an enantiomeric excess of over 99% and a conversion rate of up to 50% through direct hydrolysis of inexpensive racemic ethyl-3-hydroxybutyrate catalyzed by sin3406-1. Interestingly, protease sin3406-1 exhibited the same enantio-preference as that of esterase PHE21 during the asymmetric hydrolysis of the ester bonds of racemic ethyl-3-hydroxybutyrate. Through mutation studies and molecular docking, we also demonstrated that the four residues close to the catalytic center, S85, A86, Q87 and Y254, played key roles in both the hydrolytic activity and the enantioselectivity of protease sin3406-1, possibly through forming hydrogen bonds between the enzyme and the substrates. Deep-sea microbial proteases represented by sin3406-1 are new contributions to the biocatalyst library for the preparation of valuable chiral drug intermediates and chemicals through enzymatic kinetic resolution.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17642]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Shandong Univ Tradit Chinese Med, Affiliated Hosp, Jinan 250014, Shandong, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Materia Med, Guangzhou 510301, Guangdong, Peoples R China 4.Guangxi Normal Univ, Coll Life Sci, Guilin 541004, Peoples R China 5.South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yunfeng,Huang, Jinlong,Xu, Yongkai,et al. Utilization of one novel deep-sea microbial protease sin3406-1 in the preparation of ethyl (S)-3-hydroxybutyrate through kinetic resolution[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2018,34(9):124.
APA	Hu, Yunfeng,Huang, Jinlong,Xu, Yongkai,Zhang, Yun,&Sun, Aijun.(2018).Utilization of one novel deep-sea microbial protease sin3406-1 in the preparation of ethyl (S)-3-hydroxybutyrate through kinetic resolution.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,34(9),124.
MLA	Hu, Yunfeng,et al."Utilization of one novel deep-sea microbial protease sin3406-1 in the preparation of ethyl (S)-3-hydroxybutyrate through kinetic resolution".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 34.9(2018):124.