Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress
	Luo, Peng ; Huang, Wen 4; Li, Hongmei 3; Cheng, Chuhang 3; Ren, Chunhua 4; Chen, Ting 4; Jiang, Xiao 4; Cheng, Kaimin 1; Hu, Chaoqun 3,4
刊名	PLOS ONE
	2018
卷号	13 期号:12 页码:e0207771
ISSN号	1932-6203
英文摘要	Soil salinization. erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 +/- 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), antilipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins "Polymerase (RNA) II (DNA directed) polypeptide A" (POLR2A), "Histone acetyltransferase p300" (EP300) and "Heat shock 70kDa protein 8 '' (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins "Heat shock 70kDa protein 4 '' (HSPA4), "FBJ murine osteosarcoma viral oncogene homolog" (FOS) and "Nucleoporin 54kDa" (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of "EP300-HSPA8" and "HSPA4-NUP54" were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17595]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Yuehai Feed Grp Co LTD, Zhanjiang, Peoples R China 3.South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Luo, Peng,Huang, Wen,Li, Hongmei,et al. Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress[J]. PLOS ONE,2018,13(12):e0207771.
APA	Luo, Peng.,Huang, Wen.,Li, Hongmei.,Cheng, Chuhang.,Ren, Chunhua.,...&Hu, Chaoqun.(2018).Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress.PLOS ONE,13(12),e0207771.
MLA	Luo, Peng,et al."Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress".PLOS ONE 13.12(2018):e0207771.