Comparative transcriptome analysis reveals the regulatory networks of cytokinin in promoting the floral feminization in the oil plant Sapium sebiferum | |
Ni, Jun1; Shah, Faheem Afzal2; Liu, Wenbo1; Wang, Qiaojian2; Wang, Dongdong2; Zhao, Weiwei1; Lu, Weili1; Huang, Shengwei1; Fu, Songling2; Wu, Lifang1 | |
刊名 | BMC PLANT BIOLOGY |
2018-05-30 | |
卷号 | 18期号:无页码:16 |
关键词 | 6-BA TDZ Floral feminization Bioenergy plant Sapium sebiferum |
ISSN号 | 1471-2229 |
DOI | 10.1186/s12870-018-1314-5 |
英文摘要 | Background: Sapium sebiferum, whose seeds contain high level of fatty acids, has been considered as one of the most important oil plants. However, the high male to female flower ratio limited the seed yield improvement and its industrial potentials. Thus, the study of the sex determination in S. sebiferum is of significant importance in increasing the seed yield. Results: In this study, we demonstrated that in S. sebiferum, cytokinin (CK) had strong feminization effects on the floral development. Exogenous application with 6-benzylaminopurine (6-BA) or thidiazuron (TDZ) significantly induced the development of female flowers and increased the fruit number. Interestingly, the feminization effects of cytokinin were also detected on the androecious genotype of S. sebiferum which only produce male flowers. To further investigate the mechanism underlying the role of cytokinin in the flower development and sex differentiation, we performed the comparative transcriptome analysis of the floral buds of the androecious plants subjected to 6-BA. The results showed that there were separately 129, 352 and 642 genes differentially expressed at 6 h, 12 h and 24 h after 6-BA treatment. Functional analysis of the differentially expressed genes (DEGs) showed that many genes are related to the hormonal biosynthesis and signaling, nutrients translocation and cell cycle. Moreover, there were twenty one flowering-related genes identified to be differentially regulated by 6-BA treatment. Specifically, the gynoecium development-related genes SPATULA (SPT), KANADI 2 (KAN2), JAGGED (JAG) and Cytochrome P450 78A9 (CYP79A9) were significantly up-regulated, whereas the expression of PISTILLATA (PI), TATA Box Associated Factor II 59 (TAFII59) and MYB Domain Protein 108 (MYB108) that were important for male organ development was down-regulated in response to 6-BA treatment, demonstrating that cytokinin could directly target the floral organ identity genes to regulate the flower sex. Conclusions: Our work demonstrated that cytokinin is a potential regulator in female flower development in S. sebiferum. The transcriptome analysis of the floral sex transition from androecious to monoecious in response to cytokinin treatment on the androecious S. sebiferum provided valuable information related to the mechanism of sex determination in the perennial woody plants. |
资助项目 | Science and Technology Service program of Chinese Academy of Sciences[KFJ-STS-ZDTP-002] ; major special project of Anhui Province[16030701103] ; key program of 13th five-year plan, CASHIPS[kp-2017-21] ; National Natural Science Foundation of China[31500531] ; Natural Science Foundation of Anhui Province[1708085QC70] ; Grant of the President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences[YZJJ201619] |
WOS关键词 | ABSCISIC-ACID BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; JATROPHA-CURCAS ; CELL-DIVISION ; PLUKENETIA-VOLUBILIS ; INFLORESCENCE BUDS ; SEX EXPRESSION ; UP-REGULATION ; GENE ; AUXIN |
WOS研究方向 | Plant Sciences |
语种 | 英语 |
出版者 | BIOMED CENTRAL LTD |
WOS记录号 | WOS:000433994600001 |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/36836] |
专题 | 合肥物质科学研究院_技术生物与农业工程研究所 |
通讯作者 | Wu, Lifang |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Anhui Agr Univ, Sch Forestry & Landscape Architecture, Hefei, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Ni, Jun,Shah, Faheem Afzal,Liu, Wenbo,et al. Comparative transcriptome analysis reveals the regulatory networks of cytokinin in promoting the floral feminization in the oil plant Sapium sebiferum[J]. BMC PLANT BIOLOGY,2018,18(无):16. |
APA | Ni, Jun.,Shah, Faheem Afzal.,Liu, Wenbo.,Wang, Qiaojian.,Wang, Dongdong.,...&Wu, Lifang.(2018).Comparative transcriptome analysis reveals the regulatory networks of cytokinin in promoting the floral feminization in the oil plant Sapium sebiferum.BMC PLANT BIOLOGY,18(无),16. |
MLA | Ni, Jun,et al."Comparative transcriptome analysis reveals the regulatory networks of cytokinin in promoting the floral feminization in the oil plant Sapium sebiferum".BMC PLANT BIOLOGY 18.无(2018):16. |
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