| Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp D4 and cloning of its cnb genes | |
| Wu, Zhiguo1; Liu, Yalong1; Zhang, Ji1; Shen, Weiliang1; Lu, Wenxiang1; Hong, Qing1,2; Li, Shunpeng1 | |
| 刊名 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
 |
| 2011-09-01 | |
| 卷号 | 65期号:6页码:871-876 |
| 关键词 | 4-Chloronitrobenzene (4-CNB) Degradation Cupriavidus Heavy metal-resistance |
| ISSN号 | 0964-8305 |
| DOI | 10.1016/j.ibiod.2011.06.007 |
| 通讯作者 | Hong, Qing(hongqingnjau@yahoo.com.cn) |
| 英文摘要 | Strain D4 was isolated from the sludge of the wastewater treating system of a 4-Chloronitrobenzene (4-CNB) manufacturer. It was able to utilize 4-CNB as the sole carbon and nitrogen source for growth. Strain D4 was preliminarily identified as Cupriavidus sp. based on its physiological & biochemical characteristics and 16S rRNA gene sequence analysis. It could completely degrade 300 mg L(-1) of 4-CNB within 25 h under the condition of 30 degrees C and pH 7.0. Strain D4 could also degrade 4-CNB in presence of heavy metals including Co(2+), Cd(2+), Pb(2+), Zn(2+), Mn(2+) and so on, therefore it was an excellent candidate for the bio-treatment of 4-CNB and heavy metals co-contaminated environments. The main 4-CNB degrading related genes (cnb A, B, Cab, D, C. Z) and arsenate resistance gene fragment of strain D4 were cloned, sequenced and analyzed, which showed high similarity with the corresponding genes of a reported 4-CNB-degrader, strain CNB-1. The cnb genes of strain D4 were located on two plasmids. This is the first report on the degradation of 4-CNB by the strain from the genus of Cupriavidus sp. (C) 2011 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | PSEUDOMONAS-PUTIDA ZWL73 ; SP STRAIN CNB-1 ; NITROBENZENE DEGRADATION ; INITIAL REACTIONS ; BIODEGRADATION ; CONSTRUCTION ; SEQUENCE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| WOS类目 | Biotechnology & Applied Microbiology ; Environmental Sciences |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000295350500019 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2558999 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Hong, Qing |
| 作者单位 | 1.Nanjing Agr Univ, Coll Life Sci, Minist Agr, Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Zhiguo,Liu, Yalong,Zhang, Ji,et al. Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp D4 and cloning of its cnb genes[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2011,65(6):871-876. |
| APA | Wu, Zhiguo.,Liu, Yalong.,Zhang, Ji.,Shen, Weiliang.,Lu, Wenxiang.,...&Li, Shunpeng.(2011).Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp D4 and cloning of its cnb genes.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,65(6),871-876. |
| MLA | Wu, Zhiguo,et al."Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp D4 and cloning of its cnb genes".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 65.6(2011):871-876. |
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