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	Characterization of recombinant human granulocyte colony stimulating factor (rHuG-CSF) by capillary zone electrophoresis, capillary isoelectric focusing electrophoresis and electrospray ionization mass spectrometry
	Zhou, GH; Luo, GA; Sun, GQ; Cao, YC; Zhang, XD; Zhang, X
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2004-05-28
卷号	35期号:3页码:425-432
关键词	recombinant human granulocyte colony-stimulating factor capillary zone electrophoresis capillary isoelectric focusing electrophoresis electrospray ionization mass spectrometry glycosylated forms
ISSN号	0731-7085
DOI	10.1016/j.jpba.2004.02.003
通讯作者	Zhou, GH(ghzhou@public1.ptt.js.cn)
英文摘要	Recombinant human granulocyte colony-stimulating factor (rHuG-CSF) is a hematopietic cytokine that stimulates and regulates the proliferation and differentiation of neutrophils. Glycosylated and non-glycosylated forms of rHuG-CSF cannot be distinguished by traditional biological assays. In addition, it is very difficult to characterize impurities of the same molecular weight in biologicals. In this study, non-glycosylated rHuG-CSF, two glycosylated rHuG-CSF isoforms and their commercial dosages were successfully separated by capillary zone electrophoresis (CZE) using 50 mM Tricine containing 20 mM NaCl and 2.5 mM 1,4-diaminobutane (DAB) at pH 8.0, which could be employed for the qualitative discrimination assay of rHuG-CSF related products. CZE, capillary isoelectric focusing electrophoresis (CIEF), and mass spectrometry (MS) were used to effectively characterize non-glycosylated rHuG-CSF. It was found that proteins in the samples with different pIs in the CIEF profile could not be detected by CZE, while no difference was observed between these proteins and rHuG-CSF. Further analysis by electrospray ionization mass spectrometry with the resolution of 2000 showed that the components with different pIs in the non-glycosylated rHuG-CSF bulk sample are nearly equal in molecular weight. Therefore, it is necessary to combine several modem analytical techniques for quality control to get well-characterized biologicals. (C) 2004 Elsevier B.V. All rights reserved.
收录类别	SCI
WOS关键词	LIQUID-CHROMATOGRAPHY ; GLYCOFORMS ; PROTEINS ; ERYTHROPOIETIN ; SEPARATION ; ISOFORMS ; GEL
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
WOS类目	Chemistry, Analytical ; Pharmacology & Pharmacy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000221710100002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2558443
专题	南京土壤研究所
通讯作者	Zhou, GH
作者单位	1.Huadong Res Inst Med & Biotech, Nanjing 210002, Jiangsu, Peoples R China 2.Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Nanjing 210018, Peoples R China
推荐引用方式 GB/T 7714	Zhou, GH,Luo, GA,Sun, GQ,et al. Characterization of recombinant human granulocyte colony stimulating factor (rHuG-CSF) by capillary zone electrophoresis, capillary isoelectric focusing electrophoresis and electrospray ionization mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2004,35(3):425-432.
APA	Zhou, GH,Luo, GA,Sun, GQ,Cao, YC,Zhang, XD,&Zhang, X.(2004).Characterization of recombinant human granulocyte colony stimulating factor (rHuG-CSF) by capillary zone electrophoresis, capillary isoelectric focusing electrophoresis and electrospray ionization mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,35(3),425-432.
MLA	Zhou, GH,et al."Characterization of recombinant human granulocyte colony stimulating factor (rHuG-CSF) by capillary zone electrophoresis, capillary isoelectric focusing electrophoresis and electrospray ionization mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 35.3(2004):425-432.

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