QQ客服 官方微博 反馈留言

	Development of an HPLC-DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts
	Xin, Aiyi1,2,3; Zhang, Yaming1,2,3; Zhang, Yanxia1,2,3; Di, Duolong1,2; Liu, Junxi1,2
刊名	BIOMEDICAL CHROMATOGRAPHY
	2018-10-01
卷号	32期号:10页码:7
关键词	HPLC-DAD method isoquinoline alkaloids liquid-liquid extraction quantitative determination Stephania yunnanensis Lo
ISSN号	0269-3879
DOI	10.1002/bmc.4292
通讯作者	Liu, Junxi(liujx@licp.cas.cn)
英文摘要	For the rational utilization and the quantitative quality control of the Stephania yunnanensis Lo, an HPLC-DAD method was developed for the quantitative and simultaneous determination of five alkaloids in rat plasma (stepharine, sinomenine, palmatine, isocorydine and tetrahydropalmatine), which were the main active chemical constituents of this plant and belong to four kinds of isoquinoline-type alkaloids (protoberberine, morphine, aporphine and protaporphine alkaloids). The contents of five alkaloids ranged from 0.09 to 2.32% (w/w). The method validation was tested for the linearity (r(2)>0.9975), precision (intra-day RSD<4.8% and inter-day RSD<4.9%), extraction recovery (85.49 +/- 2.29% to 99.21 +/- 1.48%) and stability (98.5 +/- 5.3% to 101.2 +/- 3.4%). We developed an HPLC-DAD method to simultaneously measure these alkaloids in rat plasma after oral administration of the extract of this plant to rats. The results supported the hypothesis that isoquinoline alkaloids were the compounds responsible for the main pharmacological activities for anti-inflammatory and analgesic.
收录类别	SCI
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; HEPATOCELLULAR-CARCINOMA ; ISOCORYDINE DERIVATIVES ; ISOQUINOLINE ALKALOIDS ; SEPARATION ; SINOMENINE ; APOPTOSIS ; ACID
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY
WOS记录号	WOS:000445175600009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2558305
专题	寒区旱区环境与工程研究所
通讯作者	Liu, Junxi
作者单位	1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xin, Aiyi,Zhang, Yaming,Zhang, Yanxia,et al. Development of an HPLC-DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts[J]. BIOMEDICAL CHROMATOGRAPHY,2018,32(10):7.
APA	Xin, Aiyi,Zhang, Yaming,Zhang, Yanxia,Di, Duolong,&Liu, Junxi.(2018).Development of an HPLC-DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts.BIOMEDICAL CHROMATOGRAPHY,32(10),7.
MLA	Xin, Aiyi,et al."Development of an HPLC-DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts".BIOMEDICAL CHROMATOGRAPHY 32.10(2018):7.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。