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	The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation
	Hu, Wei; Chen, Jihong; Wu, Qinghua; Li, Wenjian; Liu, Jing; Lu, Dong; Wang, Shuyang
刊名	BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
	2017
卷号	60页码:12
关键词	L. thermophilus mutant heavy ion irradiation L-(+)-lactic acid production carbon source fermentation characteristic
ISSN号	1516-8913
DOI	10.1590/1678-4324-2016160337
通讯作者	Wang, Shuyang(wangsy@impcas.ac.cn)
英文摘要	Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production by mutant SRZ50 was both the highest, respectively, 23.16 +/- 0.72 g/L or 23.24 +/- 0.66 g/L, which had a significant increase from that of wild type (P<0.01), following obvious increase in biomass (P<0.05). When yeast powder was the sole nitrogen source, it can promote mutant SRZ50 to accumulate the highest L-(+)-lactic acid accumulation, which also had a significant increase from that of wild type (P<0.01). Under different fermentation periods, it was obtained that mutant SRZ50 all exhibited significant increase in L-(+)-lactic acid accumulation from wild type. In conclusion, a mutant strain with improved production profiles for L-(+)-lactic acid, was obtained, indicating that heavy ions can be an efficient tool to improve metabolic product accumulations in microbes.
收录类别	SCI
WOS关键词	DIFFERENT NITROGEN-SOURCES ; LACTIC-ACID ; L(+)-LACTIC ACID ; RHIZOPUS-ORYZAE ; ARABIDOPSIS-THALIANA ; STRAIN IMPROVEMENT ; ASPERGILLUS-NIGER ; BEAM IRRADIATION ; CARBON-SOURCES ; CITRIC-ACID
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
语种	英语
出版者	INST TECNOLOGIA PARANA
WOS记录号	WOS:000435656500001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2557806
专题	寒区旱区环境与工程研究所
通讯作者	Wang, Shuyang
作者单位	Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Hu, Wei,Chen, Jihong,Wu, Qinghua,et al. The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation[J]. BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,2017,60:12.
APA	Hu, Wei.,Chen, Jihong.,Wu, Qinghua.,Li, Wenjian.,Liu, Jing.,...&Wang, Shuyang.(2017).The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation.BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,60,12.
MLA	Hu, Wei,et al."The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation".BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY 60(2017):12.

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