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	Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins
	Qiao, Jianyu1,2,3; Gao, Ruibin4; Wang, Zhongyang1,2,3; Sun, Yaming1,2,3; Dong, Shuqing1,2; Zhao, Liang1,2
刊名	EMIRATES JOURNAL OF FOOD AND AGRICULTURE
	2019-03-01
卷号	31期号:3页码:164-171
关键词	Capillary electrophoresis Amino acids Derivatization Jujube
ISSN号	2079-052X
DOI	10.9755/ejfa.2019.v31.i3.1928
通讯作者	Dong, Shuqing(sqdong@licp.cas.cn) ; Zhao, Liang(zhaol@licp.cas.cn)
英文摘要	A simple capillary electrophoresis (CE) method that using beta-cyclodextrin (beta-CD) as buffer modifier has been developed for the detection of ten amino acids of jujube from five different geographical origins. The CE methodology was optimized through the variation of type, pH and concentration of the buffer solution, concentration of the additive beta-CD, applied voltage, injection time and wavelength of the UV detection. It was found that the best separation of ten kinds of amino acids was achieved within less than 6 min under the optimum separation conditions: 50 mmol L-1 pH 9.5 borate-phosphate buffer with 5.0 mmol L-1 beta-CD, 15 kV applied voltage, 25 degrees C column temperature, 210 nm detection wavelength, and 5 s injection time. This method showed good repeatability with RSD values of 1.4-3.4% for peak area, and 1.4-4.7% for migration time, when beta-CD was used as buffer modifier. Under the optimum conditions, the method has been successfully applied to the detection of actual jujube samples, which also verifies the effectiveness and practicability of the method. Recovery of real samples was in ranging of 90%-105%, which proved the feasibility of the method. It also proved that the method was successfully applied to the quantitative analysis of amino acids of interest in plant samples.
收录类别	SCI
WOS关键词	ZIZIPHUS-JUJUBA ; ZIZYPHUS-JUJUBA ; POLYSACCHARIDES ; FLAVONOIDS ; COMPLEXES ; SAPONINS ; FRUITS
WOS研究方向	Agriculture ; Food Science & Technology
WOS类目	Agronomy ; Food Science & Technology
语种	英语
出版者	UNITED ARAB EMIRATES UNIV
WOS记录号	WOS:000468204100003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2555460
专题	寒区旱区环境与工程研究所
通讯作者	Dong, Shuqing; Zhao, Liang
作者单位	1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China 4.Third Prov Peoples Hosp Gansu, Dept Pharm, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Jianyu,Gao, Ruibin,Wang, Zhongyang,et al. Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins[J]. EMIRATES JOURNAL OF FOOD AND AGRICULTURE,2019,31(3):164-171.
APA	Qiao, Jianyu,Gao, Ruibin,Wang, Zhongyang,Sun, Yaming,Dong, Shuqing,&Zhao, Liang.(2019).Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins.EMIRATES JOURNAL OF FOOD AND AGRICULTURE,31(3),164-171.
MLA	Qiao, Jianyu,et al."Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins".EMIRATES JOURNAL OF FOOD AND AGRICULTURE 31.3(2019):164-171.

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