Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs

doi:10.1039/c9ra02069a

	Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs
	Jing, Bolin 1,2; Luo, Yong 3; Lin, Bingcheng 4; Li, Jianjun 1; Wang, Zhuo A.1; Du, Yuguang 1
刊名	RSC ADVANCES
	2019-06-03
卷号	9 期号:30 页码:17137-17147
ISSN号	2046-2069
DOI	10.1039/c9ra02069a
英文摘要	Tumor metastasis is one of the main causes of cancer-related death, and it is difficult to study the whole process of tumor metastasis in vivo due to the complex physiological environment in the body. Therefore, it's crucial to develop simple and physiologically relevant in vitro cancer models to study the metastasis process, especially different phases of tumor metastasis. A novel microfluidic tumor-vessel co-culture system was established to reproduce the different phases of cancer metastasis (proliferation, migration, intravasation and adherence) individually in vitro for the first time. It was observed that blood vessels with fluid flow had big impact on metastasis of liver cancer cells HepG2 and breast ones MDA-MB-231. In particular, it was found that both HepG2 and MDA-MB-231 cells migrated in the direction of "blood flow". Furthermore, MDA-MB-231 cells invaded through paracellular mode disrupting the intercellular endothelial junctions, whereas HepG2 cells engaged in transcellular intravasation through transcellular process. Compared with traditional assays, much more potent inhibition of 5-fluorouracil (5-Fu) on different phases of tumor metastasis was observed on the microsystem. In summary, the microfluidic device yielded abundant information about each phase of tumor metastasis, and would provide a powerful platform for use in drug screening, toxicology studies, and personalized medicine in future.
资助项目	National Natural Science Foundation of China[31870802]
WOS关键词	ENDOTHELIAL BARRIER ; MICROFLUIDIC DEVICE ; INTERSTITIAL FLOW ; CELL INVASION ; CANCER ; MODEL ; MIGRATION ; MICROENVIRONMENT ; PERMEABILITY ; STROMA
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000471912700022
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/30058]
专题	中国科学院过程工程研究所
通讯作者	Li, Jianjun; Wang, Zhuo A.; Du, Yuguang
作者单位	1.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA,Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Jing, Bolin,Luo, Yong,Lin, Bingcheng,et al. Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs[J]. RSC ADVANCES,2019,9(30):17137-17147.
APA	Jing, Bolin,Luo, Yong,Lin, Bingcheng,Li, Jianjun,Wang, Zhuo A.,&Du, Yuguang.(2019).Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs.RSC ADVANCES,9(30),17137-17147.
MLA	Jing, Bolin,et al."Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs".RSC ADVANCES 9.30(2019):17137-17147.