Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation

doi:10.1016/j.colsurfb.2019.03.053

	Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation
	Liaw, Bor-Shuang 1; Xing, Fangyu 2; Wang, Dan 1; Gao, Fei 3; Lu, Jingsong 4; Yu, Jing 4; Sun, Xiaodan 1; Wang, Xiumei 1; Feng, Qingling 1; Zhang, Guifeng 2
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2019-07-01
卷号	179 页码:48-55
关键词	Collagen fibrillogenesis Langmuir-Blodgett deposition Self-assembly Cell adhesion Cell proliferation
ISSN号	0927-7765
DOI	10.1016/j.colsurfb.2019.03.053
英文摘要	Collagen fibrillogenesis is of special significance for the maintenance of collagen scaffold's mechanical stability and biological performance. Comprehensive information about the mechanism of collagen fibrillogenesis in vitro, as well as the effect of fibrillogenesis on deposited layers of ordered collagen molecules for cellular behavior regulation is thus crucial. In the current study, the pH, phosphate ion as well as reconstitution time impacting on the in vitro fibrillogenesis was systematically investigated, including the zeta potential and turbidity measurement. Furthermore, the fibrillogenesis impacting on the pi-a isotherms of collagen assembly at the air/water interface was then fully evaluated. By applying LB technique, collagen fibril-assembling arrays structure can be successfully transferred to form surface deposition onto the mica and glass substrate. The morphology and collagen content were subsequently assessed by atomic force microscopy (AFM) and hydrolyzing examination respectively. Effect of collagen LB deposition on the adhesion and proliferation of SD rat bone marrow mesenchymal stem cells were estimated by Rhodamine Phalloidin/DIPI staining and CCK8 proliferation assays. The results show that highly oriented and collagen-abundant thin film can further facilitate cell adhesion and proliferation, indicating an innovative direction for tissue engineering.
资助项目	Intergovernmental cooperation in science and technology[2016YFE0125300] ; National Natural Science Foundation of China (NSFC)[81671829] ; National Key R&D Program of China[2018YFA0108202] ; National Key R&D Program of China[2018YFC1106402] ; Science and Technology Program of Guangzhou, China[201803010086]
WOS关键词	MOLECULAR-INTERACTIONS ; SCHAEFER FILMS ; PACLITAXEL ; ADSORPTION ; MICROSCOPY ; NETWORK ; PH
WOS研究方向	Biophysics ; Chemistry ; Materials Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000471736600006
资助机构	Intergovernmental cooperation in science and technology ; National Natural Science Foundation of China (NSFC) ; National Key R&D Program of China ; Science and Technology Program of Guangzhou, China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/30035]
专题	中国科学院过程工程研究所
通讯作者	Zhang, Guifeng; Zhao, Lingyun
作者单位	1.Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Key Lab Adv Mat,Minist Educ China, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Northwest Univ, Coll Chem & Mat Sci, Xian 710069, Shaanxi, Peoples R China 4.Zhejiang Univ Technol, Coll Mat Sci & Engn, Hangzhou 310014, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liaw, Bor-Shuang,Xing, Fangyu,Wang, Dan,et al. Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,179:48-55.
APA	Liaw, Bor-Shuang.,Xing, Fangyu.,Wang, Dan.,Gao, Fei.,Lu, Jingsong.,...&Zhao, Lingyun.(2019).Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation.COLLOIDS AND SURFACES B-BIOINTERFACES,179,48-55.
MLA	Liaw, Bor-Shuang,et al."Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation".COLLOIDS AND SURFACES B-BIOINTERFACES 179(2019):48-55.