Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus

doi:10.1016/j.biortech.2019.121486

	Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus
	Sharshar, Moustafa Mohamed 1,2; Samak, Nadia Abdrabo 1,2,3; Hao, Xuemi 1,2; Mu, Tingzhen 1; Zhong, Wei 1,2; Yang, Maohua 1; Peh, Sumit 1,2; Ambreen, Sadaf 2,4; Xing, Jianmin1,2
刊名	BIORESOURCE TECHNOLOGY
	2019-09-01
卷号	288 页码:9
关键词	Thioalkalivibrio versutus Fed-batch cultivation Bio-desulfurization Inducible regulation system Promoter engineering
ISSN号	0960-8524
DOI	10.1016/j.biortech.2019.121486
英文摘要	Highly toxic and flammable H2S gas has become an environmental threat. Because of its ability to efficiently remove H2S by oxidation, Thioalkalivibrio versutus is gaining more attention. Haloalkaliphilic autotrophs, like the bio-desulfurizing T. versutus, grow weakly. Weak growth makes any trial for developing potent genetic tools required for genetic engineering far from achieved. In this study, the fed-batch strategy improved T. versutus growth by 1.6 fold in maximal growth rate, 9-fold in 0.D-600 values and about 3-fold in biomass and protein productions. The strategy also increased the favorable desulfurization product, sulfur, by 2.7 fold in percent yield and 1.5-fold in diameter. A tight iron-inducible expression system for T. versutus was successfully developed. The system was derived from fed-batch cultivation coupled with new design, build, test and validate (DPTV) approach. The inducible system was validated by toxin expression. Fed-batch cultivation coupled with DPTV approach could be applied to other autotrophs.
资助项目	National Science Foundation of China[21878307] ; National Science Foundation of China[31800030] ; National Science Foundation of China[31872633] ; CAS-TWAS (The World Academy of Sciences) President's Fellowship for International PhD students
WOS关键词	FED-BATCH CULTIVATION ; ESCHERICHIA-COLI ; SYNTHETIC BIOLOGY ; GENE-EXPRESSION ; PERFORMANCE ; VECTORS ; SULFIDE ; IRON ; TRANSLATION ; PROTEINS
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000472018100041
资助机构	National Science Foundation of China ; CAS-TWAS (The World Academy of Sciences) President's Fellowship for International PhD students
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/29955]
专题	中国科学院过程工程研究所
通讯作者	Xing, Jianmin
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt 4.Chinese Acad Sci, Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabo,Hao, Xuemi,et al. Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2019,288:9.
APA	Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabo.,Hao, Xuemi.,Mu, Tingzhen.,Zhong, Wei.,...&Xing, Jianmin.(2019).Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,288,9.
MLA	Sharshar, Moustafa Mohamed,et al."Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 288(2019):9.