Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides

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	Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides
	Liu, YS; Zhang, J; Liu, Q; Zhang, CG; Ma, QS; Liu, YS, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China
刊名	CURRENT MICROBIOLOGY
	2004
卷号	49 期号:4 页码:234-238
ISSN号	0343-8651
英文摘要	A thermophilic bacterial strain GXN151 which could degrade Avicel efficiently was isolated and identified as Bacillus licheniformis. A genomic library of GXN151 was constructed and two novel endoglucanase genes designated cel9A and cel12A were isolated by screening the library on carboxylmethyl cellulase indicator plates. The analysis of amino acid sequences deduced from the genes indicated that Cel9A consisted of a catalytic domain belonging to glycosyl hydrolase family 9, a linker domain, and a carbohydrate binding module family 3 from N-terminal to C-terminal; Cel12A had only one catalytic domain belonging to glycosyl hydrolase family 12. The combinations of Cel9A and Cel12A produced by the recombinant E. coli exhibited synergistic action against substrates of carboxylmethyl cellulose as well as Avicel.
学科主题	Microbiology
语种	英语
WOS记录号	WOS:000224199500002
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55247]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Liu, YS, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China
推荐引用方式 GB/T 7714	Liu, YS,Zhang, J,Liu, Q,et al. Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides[J]. CURRENT MICROBIOLOGY,2004,49(4):234-238.
APA	Liu, YS,Zhang, J,Liu, Q,Zhang, CG,Ma, QS,&Liu, YS, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China.(2004).Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides.CURRENT MICROBIOLOGY,49(4),234-238.
MLA	Liu, YS,et al."Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides".CURRENT MICROBIOLOGY 49.4(2004):234-238.