Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity

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	Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity
	Cui, JX; Ji, JF; Lv, AG; Wu, WF; Wu, WF, Chinese Acad Sci, Inst Appl Ecol, 72,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China
刊名	BIOCHEMISTRY-MOSCOW
	2005
卷号	70 期号:1 页码:62-68
关键词	Immunotoxin Interleukin-4 Pseudomonas Exotoxin a
ISSN号	0006-2979
英文摘要	Interleukkin-4 receptors (IL-4Rs) are expressed on a wide variety of human cancer cells, and therefore it maybe a good option to treat IL-4R-bearing tumors with IL-4-fusing immunotoxins. In this study, the gene encoding human interleukin-4 mutein cpIL-4(13D) was obtained through overlapping polymerase chain reaction. A chimeric immunotoxin was constructed by genetically fusing the mutein cpIL-4(13D) to a modified version of Pseudomonas exotoxin A (PE38KDEL) and was expressed in Escherichia coli AD494 (DE3). The expression level of the fusion protein was about 30% of the total bacterial protein assessed by SDS-PAGE analysis. After purification by affinity chromatography and anion exchange chromatography, the chimeric protein was tested for its cytotoxicity. Our data show that cpIL-4(13D)-PE38KDEL has improved cytotoxicity on IL-4R-bearing tumor cells in comparison with other IL-4-fusing immunotoxins and might be useful in treating tumors with a large number of IL-4Rs.
学科主题	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000227139600006
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55187]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Wu, WF, Chinese Acad Sci, Inst Appl Ecol, 72,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Cui, JX,Ji, JF,Lv, AG,et al. Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity[J]. BIOCHEMISTRY-MOSCOW,2005,70(1):62-68.
APA	Cui, JX,Ji, JF,Lv, AG,Wu, WF,&Wu, WF, Chinese Acad Sci, Inst Appl Ecol, 72,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China.(2005).Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity.BIOCHEMISTRY-MOSCOW,70(1),62-68.
MLA	Cui, JX,et al."Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity".BIOCHEMISTRY-MOSCOW 70.1(2005):62-68.