A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes

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	A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes
	Wu, ZL; Xie, LP; Xia, GL; Zhang, JF; Nie, YC; Hu, JC; Wang, SJ; Zhang, RQ; Zhang, RQ, Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
刊名	TOXICON
	2005
卷号	45 期号:7 页码:851-859
关键词	Actinomycete Nocardiopsis Dassonvillei Tetrodotoxin Fugu Rubripes
ISSN号	0041-0101
英文摘要	Three puffer fishes, Fugu rubripes, collected from the Bohai Sea of China were examined for tetrodotoxin-producing microorganisms. An actinomycete isolated from the ovaries of the puffer fishes was found to produce tetrodotoxin. After being cultured at 28 degrees C for 7 days, cells were harvested by centrifuge and disrupted by ultrasonication. The toxin was purified from the cell lyzate by ultrafiltration, active charcoal column, Bio-gel-p2 and ion exchange column chromatography. Mouse neuroblastoma cell culture, thin-layer chromatography, fluorimetric spectrophotometry, UV-spectrophotometry and electrospray ionization mass spectrometry, together with mouse bioassay demonstrated that the isolated strain produced tetrodotoxin and related toxin during cultivation. Based on morphological, physiological, biochemical characteristics and 16S rDNA alignment, this strain was identified as Nocardiopsis dassonvillei. Our findings suggested that N. dassonvillei in the ovaries was closely related to the toxification of the puffer fish. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy ; Toxicology
语种	英语
WOS记录号	WOS:000229846500005
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55129]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhang, RQ, Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Wu, ZL,Xie, LP,Xia, GL,et al. A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes[J]. TOXICON,2005,45(7):851-859.
APA	Wu, ZL.,Xie, LP.,Xia, GL.,Zhang, JF.,Nie, YC.,...&Zhang, RQ, Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China.(2005).A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes.TOXICON,45(7),851-859.
MLA	Wu, ZL,et al."A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes".TOXICON 45.7(2005):851-859.