Study of microbial community structures in UASB sludge treating municipal wastewater by denaturing gradient gel electrophoresis of 16S rDNA

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	Study of microbial community structures in UASB sludge treating municipal wastewater by denaturing gradient gel electrophoresis of 16S rDNA
	Zhang, Y; Aiyuk, S; Xu, H; Chen, GX; Verstraete, W; Zhang, Y, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2005
卷号	48 页码:128-135
关键词	Uasb Municipal Wastewater Anaerobic Sludge Community Analysis Dgge
ISSN号	1006-9305
英文摘要	The structures of microbial communities in lab-scale upflow anaerobic sludge blanket (UASB) reactors for treating municipal wastewater with different ratios of COD (soluble)/COD total were studied using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes. The microbial structure of the inoculum sludge obtained from a full-scale UASB reactor of treating potato processing wastewater was compared with the structures of sludges collected from three lab-scale UASB reactors after eight months feeding with raw municipal wastewater, with CEPS (chemically enhanced primary sedimentation) pretreated municipal wastewater, and with a synthetic municipal sewage, respectively. Computer-aided numerical analysis of the DGGE fingerprints showed that the bacterial community underwent major changes. The sludges for treating raw and CEPS pretreated wastewater had very similar bacterial and archaeal communities (82% and 96% similarity) but were different from that for treating the synthetic sewage. Hence, despite similar % COD in the particulate form in the synthetic and the real wastewater, the two wastewaters were selected for different microbial communities. Prominent DGGE bands of Bacteria and Archaea were purified and sequenced. The 16S rRNA gene sequences of the dominant archaeal bands found in the inoculum, and UASB sludge fed with raw sewage, CEPS pretreated wastewater, and synthetic sewage were closely associated with Methanosaeta concilli. In the UASB sludge fed with synthetic sewage, another dominant band associated with an uncultured archaeon 39-2 was found together with M. concilli.
学科主题	Biology
语种	英语
WOS记录号	WOS:000230897500017
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55099]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhang, Y, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Y,Aiyuk, S,Xu, H,et al. Study of microbial community structures in UASB sludge treating municipal wastewater by denaturing gradient gel electrophoresis of 16S rDNA[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2005,48:128-135.
APA	Zhang, Y,Aiyuk, S,Xu, H,Chen, GX,Verstraete, W,&Zhang, Y, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2005).Study of microbial community structures in UASB sludge treating municipal wastewater by denaturing gradient gel electrophoresis of 16S rDNA.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,48,128-135.
MLA	Zhang, Y,et al."Study of microbial community structures in UASB sludge treating municipal wastewater by denaturing gradient gel electrophoresis of 16S rDNA".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 48(2005):128-135.