Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin

CORC > 沈阳应用生态研究所 > 中国科学院沈阳应用生态研究所 > 沈阳应用生态研究所

	Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin
	Jiang, L; Feng, J; Wu, WF; Yang, DL; Yan, XY; Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2005
卷号	32 期号:9 页码:850-856
关键词	Anti-her2/neu Scfv C6.5 Htnf-alpha Immunotoxin
ISSN号	1000-3282
英文摘要	HER2/neu is an attractive target for tumor therapy since its overexpression in a number of tumors. In order to enhance the cytotoxicity of anti-HER2/neu antibody, and the specificity of TNF-alpha for tumor cell, a fusion gene of antiHER2-hTNF-alpha was constructed. The recombinant fusion protein was produced in E.coli, and their refolding has done through affinity chromatography on a His-column. The ELISA assay showed that antiHER2-hTNF-alpha specifically bound to HER2/neu expressing SKOV-3 and MCF-7 cells, but it did not recognize the HER2/neu negative cells A375. In vitro study, it was found that antiHER2-hTNF-alpha inhibited the proliferation of SKOV-3 and MCF-7 cells, whereas it did not effect on the A375. These studies suggest that the recombinant antiHER2-hTNF-alpha immunotoxin has a potential application in HER2/neu expressing tumor therapy.
学科主题	Biochemistry & Molecular Biology ; Biophysics
语种	中文
WOS记录号	WOS:000232115500008
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55067]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Jiang, L,Feng, J,Wu, WF,et al. Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(9):850-856.
APA	Jiang, L,Feng, J,Wu, WF,Yang, DL,Yan, XY,&Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China.(2005).Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(9),850-856.
MLA	Jiang, L,et al."Construction and functional study of a novel anti HER2/neu-hTNF-alpha immunotoxin".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.9(2005):850-856.