Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant

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	Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant
	Cao, J; Cronin, C; McLandsborough, L; Levin, RE; Levin, RE, Univ Massachusetts, Dept Food Sci, Massachusetts Agr Expt Stn, Amherst, MA 01003 USA
刊名	FOOD BIOTECHNOLOGY
	2005
卷号	19 期号:3 页码:217-226
关键词	L. Monocytogenes Pcr Rapd Type Shrimp Processing Environment Taq Polymerase Discrimination Index Random Primers
ISSN号	0890-5436
英文摘要	Ninety-nine randomly selected isolates of Listeria monocytogenes from several processing environment locations, in a shrimp processing plant, obtained during a 5-month sampling period were subjected to randomly amplified polymorphic DNA (RAPD) analysis with the use of four primers. Preliminary studies indicated that the number of DNA bands and their intensity differed greatly with respect to the commercial source of the Taq polymerase used with individual isolates. Eighteen composite RAPD types were discerned with the use of the four primers. Among these 18 composite RAPD types, type 1 comprised 14 indistinguishable isolates, and type 9 comprised 49 indistinguishable isolates. These results indicate that the shrimp processing plant was dominated by these 2 RAPD types that comprised 63.6% of the 99 randomly selected isolates.
学科主题	Biotechnology & Applied Microbiology ; Food Science & Technology
语种	英语
WOS记录号	WOS:000234407200005
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55025]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Levin, RE, Univ Massachusetts, Dept Food Sci, Massachusetts Agr Expt Stn, Amherst, MA 01003 USA
推荐引用方式 GB/T 7714	Cao, J,Cronin, C,McLandsborough, L,et al. Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant[J]. FOOD BIOTECHNOLOGY,2005,19(3):217-226.
APA	Cao, J,Cronin, C,McLandsborough, L,Levin, RE,&Levin, RE, Univ Massachusetts, Dept Food Sci, Massachusetts Agr Expt Stn, Amherst, MA 01003 USA.(2005).Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant.FOOD BIOTECHNOLOGY,19(3),217-226.
MLA	Cao, J,et al."Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant".FOOD BIOTECHNOLOGY 19.3(2005):217-226.