A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars

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	A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars
	He, HB; Xie, HT; Zhang, XD; Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2006
卷号	38 期号:5 页码:1083-1091
关键词	Amino Sugar C-13 N-15 Incubation Gc/ms Atom Percentage Excess (Ape)
ISSN号	0038-0717
英文摘要	Amino sugars have been used as biomarker to indicate microorganism contribution to soil organic matter turnover and sequestration. However, there is no direct gas chromatograph mass spectrometry (GUMS) approach to assess microbial synthesis of amino sugars in soil. We developed a novel method which combines laboratory incubation of substrate containing N-15 or C-13 and a GUMS technique to trace N-15 or C-13 isotope changes in three amino sugars, glucosamine, galactosamine, and muramic acid. Sample preparation followed the procedure of Zhang and Amelung (1996) [Zhang, X., Amelung, W., 1996. Gas chromatographic determination of muramic acid, glucosamine, galactosamine, and mannosamine in soils. Soil Biology and Biochemistry 28, 1201-1206.] The GUMS determination was conducted using a full scan mode with both electronic ionization (EI) and chemical ionization (0) sources. The CI source was suitable for all of the three amino sugars, while the El source was not applicable to muramic acid due to its low sensitivity in the determination as well as low concentration of muramic acid in soil. The enrichment of 15N or 13C in amino sugars during incubation was estimated by calculating the atom percentage excess (APE). 15N incorporation was evaluated according to fragment (F) abundance ratio of mass F + 1 to F, whilst C-13 incorporation was estimated according to the ratio of mass F + n to F (n is skeleton carbon number in the fragment). This novel method was assessed by using two soil samples (a Kandiudult and a Udoll) incubated with either N-15-amonium or U-C-13-glucose. The results indicate that the GUMS determination is reproducible, thus this technique is useful in detecting the microbial synthesis of amino sugars in soil, and especially it should be possible when looking at the position or how much labeled carbon and nitrogen atoms have been incorporated. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Soil Science
语种	英语
WOS记录号	WOS:000237545900025
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54965]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	He, HB,Xie, HT,Zhang, XD,et al. A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars[J]. SOIL BIOLOGY & BIOCHEMISTRY,2006,38(5):1083-1091.
APA	He, HB,Xie, HT,Zhang, XD,&Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2006).A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars.SOIL BIOLOGY & BIOCHEMISTRY,38(5),1083-1091.
MLA	He, HB,et al."A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars".SOIL BIOLOGY & BIOCHEMISTRY 38.5(2006):1083-1091.