PCR-DGGE analysis of nematode diversity in Cu-contaminated soil

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	PCR-DGGE analysis of nematode diversity in Cu-contaminated soil
	Li, Q; Wang, SB; Liang, WJ; Jiang, Y; Jiang, SW; Liang, WJ, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
刊名	PEDOSPHERE
	2008
卷号	18 期号:5 页码:621-627
关键词	Copper Contamination Morphological Analysis Nematode Diversity Pcr-dgge
ISSN号	1002-0160
英文摘要	A wheat pot experiment was conducted tinder greenhouse conditions to assess the effect of copper contamination oil soil nematode diversity by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method and morphological analysis. The soil was treated with CuSO4 center dot 5H(2)O at the following concentrations: 0, 50; 100 200 400, and 800 mg kg(-1) dry soil, and the soil samples were collected at wheat jointing and ripening stages. Nematode diversity index (H ') from morphological analysis showed no difference between the control and the treated samples in either of the sampling dates. At the wheat ripening stage, nematode diversity obtained by the PCR-DGGE method decreased noticeably in the Cu800 treatment in comparison with the control. With optimization of the method of nematode DNA extraction, PCR-DGGE could give more information oil nematode genera, and the intensity of the bands could reflect the abundance of nematode genera in the assemblage. The PCR-DGGE method proved promising in distinguishing nematode diversity in heavy metal contaminated soil.
学科主题	Soil Science
语种	英语
WOS记录号	WOS:000259219500009
资助机构	National Natural Science Foundation of China [30600087]; Chinese Academy of Sciences [06LYQY5001]
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54385]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Liang, WJ, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Li, Q,Wang, SB,Liang, WJ,et al. PCR-DGGE analysis of nematode diversity in Cu-contaminated soil[J]. PEDOSPHERE,2008,18(5):621-627.
APA	Li, Q,Wang, SB,Liang, WJ,Jiang, Y,Jiang, SW,&Liang, WJ, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2008).PCR-DGGE analysis of nematode diversity in Cu-contaminated soil.PEDOSPHERE,18(5),621-627.
MLA	Li, Q,et al."PCR-DGGE analysis of nematode diversity in Cu-contaminated soil".PEDOSPHERE 18.5(2008):621-627.