Determination of soil amino acids by high performance liquid chromatography-electro spray ionization-mass spectrometry derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate

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	Determination of soil amino acids by high performance liquid chromatography-electro spray ionization-mass spectrometry derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
	Hou, SM; He, HB; Zhang, W; Xie, HT; Zhang, XD; Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
刊名	TALANTA
	2009
卷号	80 期号:2 页码:440-447
关键词	6-aminoquinolyl-n-hydroxysuccinimidyl Carbamate High Performance Liquid Chromatography Mass Spectrometry Amino Acid Soil
ISSN号	0039-9140
英文摘要	Determination of amino acids by mass spectrometry (MS) is an important technique to investigate soil nitrogen transformation and cycling as amino acids being the major nitrogen-containing compounds in soil organic matter. However, researchers have long faced a critical problem in coupling an efficient separation technique to a sensitive MS detection system simultaneously. In this context, we established a new method of liquid chromatography coupled to mass spectrometry based on the 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization method for convenient and accurate quantification of amino acids in soil samples. Baseline separation of 17 amino acid AQC-derivatives was achieved on an XTerra(R) MS C-18 column using ammonium formate as a mobile phase modifier. The concentration of ammonium formate and the pH of the mobile phase were optimized in order to obtain sensitive MS signals. The response curves were linear over the range of 50-800 mu mol L-1 amino acids. The detection limits were 0.20-0.60 pmol mu L-1 on column and 0.07-0.24 mu g g(-1) soil under the optimized conditions. The method has been applied successfully for the first time to determine amino acids in 4 types of soil samples, in which 15 amino acids were quantified by MS detector but methionine and cystine were below the detection limits. Both the recovery and the precision were satisfactory. Hence, this proposed technique shows a potential for the identification of amino acids in soil as well as tracing the transformation of soil amino acids with isotope dilution technique in nitrogen cycling investigation. (C) 2009 Elsevier B.V. All rights reserved.
学科主题	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000271701900005
资助机构	Natural Science Foundation of China [40535028]; Major State Basic Research Development Program of China [2009CB118607]
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54009]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Hou, SM,He, HB,Zhang, W,et al. Determination of soil amino acids by high performance liquid chromatography-electro spray ionization-mass spectrometry derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate[J]. TALANTA,2009,80(2):440-447.
APA	Hou, SM,He, HB,Zhang, W,Xie, HT,Zhang, XD,&Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2009).Determination of soil amino acids by high performance liquid chromatography-electro spray ionization-mass spectrometry derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate.TALANTA,80(2),440-447.
MLA	Hou, SM,et al."Determination of soil amino acids by high performance liquid chromatography-electro spray ionization-mass spectrometry derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate".TALANTA 80.2(2009):440-447.