Study on the hydrogen production ability of high-efficiency bacteria and synergistic fermentation of maize straw by a combination of strains

doi:10.1039/c9ra00165d

	Study on the hydrogen production ability of high-efficiency bacteria and synergistic fermentation of maize straw by a combination of strains
	Bao, Hongxu 1,2,3; Zhang, Xin 1,2; Su, Hongzhi 1; Li, Liangyu 1; Lv, Zhizhong 1; Zhang, Xinyue 1
刊名	RSC ADVANCES
	2019
卷号	9 期号:16 页码:9030-9040
ISSN号	2046-2069
DOI	10.1039/c9ra00165d
英文摘要	Based on the principle of reciprocal symbiosis and co-metabolism of mixed culture microorganisms, a group of high-efficiency maize straw-degrading hydrogen-producing complex bacteria X9 + B2 was developed by a strain matching optimization experiment. Systematic research and optimization experiments were carried out on the mechanism of the main controlling factors affecting the hydrogen production of the complex bacteria. The results showed that the optimum conditions for the acid blasting pre-treatment of maize straw as a substrate were as follows: when the inoculation amount was 6% and the inoculum ratio was 1:1, at which point, we needed to simultaneously inoculate, the initial pH was 6, the substrate concentration was 12 g L-1, and the culture time was 40 h. The complex bacteria adopted the variable temperature and speed regulation hydrogen production operational mode; after the initial temperature of 37 degrees C for 8 hours, the temperature was gradually increased to 40 degrees C for 3 hours. The initial shaker speed was 90 rpm for 20 hours, and the speed was gradually increased to 130 rpm. The maximum hydrogen production rate obtained by the complex bacteria under these conditions was 12.6 mmol g(-1), which was 1.6 times that of the single strain X9 with a maximum hydrogen production rate of 5.7 mmol g(-1). Through continuous subculturing and the 10(th), 20(th), 40(th), 60(th), 80(th), 100(th) and 120(th) generation fermentation hydrogen production stability test analysis, no significant difference was observed between generations; the maximum difference was not more than 5%, indicating better functional properties and stability.
资助项目	State Key Laboratory Fund for Urban Water Environment and Water Resources[HC201814] ; State Key Program of National Natural Science of China[71731007] ; Liaoning Provincial "Innovative Talents in Colleges and Universities" Support Project[LR2016055]
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000463949500026
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/124037]
专题	中国科学院沈阳应用生态研究所
通讯作者	Bao, Hongxu
作者单位	1.Liaoning Univ, Sch Environm Sci, Shenyang 110036, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China 3.Harbin Inst Technol, State Key Lab Urban Water Resources & Environm, Harbin 150090, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Bao, Hongxu,Zhang, Xin,Su, Hongzhi,et al. Study on the hydrogen production ability of high-efficiency bacteria and synergistic fermentation of maize straw by a combination of strains[J]. RSC ADVANCES,2019,9(16):9030-9040.
APA	Bao, Hongxu,Zhang, Xin,Su, Hongzhi,Li, Liangyu,Lv, Zhizhong,&Zhang, Xinyue.(2019).Study on the hydrogen production ability of high-efficiency bacteria and synergistic fermentation of maize straw by a combination of strains.RSC ADVANCES,9(16),9030-9040.
MLA	Bao, Hongxu,et al."Study on the hydrogen production ability of high-efficiency bacteria and synergistic fermentation of maize straw by a combination of strains".RSC ADVANCES 9.16(2019):9030-9040.