Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: Bacterial community and functional gene expression mechanisms

doi:10.1016/j.chemosphere.2018.08.057

	Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: Bacterial community and functional gene expression mechanisms
	Guo, Meixia 1,2; Gong, Zongqiang 1; Miao, Renhui 3; Jia, Chunyun 1; Rookes, James 4; Cahill, David 4; Zhuang, Jie 1
刊名	CHEMOSPHERE
	2018-12-01
卷号	212 页码:15-23
关键词	Polycyclic aromatic hydrocarbons Rhizosphere effect RHD alpha gene expression Bacterial community Phytoremediation
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2018.08.057
英文摘要	To investigate the bacterial mechanisms of polycyclic aromatic hydrocarbons (PAHs) degradation in an aged-contaminated agricultural soil planted with tall fescue (Festuca arundinacea), a rhizo-box experiment was carried out for 60 d. Shifts in bacterial community structure in the soils during the experiment were performed using denaturing gradient gel electrophoresis. The abundance and activity of total bacteria and PAH-degraders were measured by quantification of 16S rDNA, PAH-ring hydroxylating dioxygenase (PAH-RHD alpha) genes and their transcripts, respectively. The residual PAH concentrations were monitored using high-performance liquid chromatography analysis. Results showed that the removal percentage of total PAHs in rhizosphere soil was 11% higher than that in unplanted soil. Soil bacteria were dominated by Alphaproteobacteria (48.4%) and Gammaproteobacteria (25.8%). Tall fescue positively affected the abundance and activity of total bacteria in the soil, and stimulated RHD alpha gram-negative (GN) gene expression while inhibiting RHD alpha gram-positive gene expression. PAH dissipation in rhizosphere soil could be ascribed to modifications in the bacterial community structure, increase in the abundance of PAH-degraders, and enhancement of the RHD alpha GN gene expression during the incubation. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	Natural Science Foundation of China[41271336] ; Natural Science Foundation of China[41673132] ; National Science Foundation for Post-doctoral of China[2018M632763]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000447478100003
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/123513]
专题	中国科学院沈阳应用生态研究所
通讯作者	Gong, Zongqiang
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Liaoning, Peoples R China 2.Henan Univ, Coll Chem & Chem Engn, Henan Joint Int Res Lab Environm Pollut Control M, Inst Environm & Analyt Sci, Kaifeng 475004, Henan, Peoples R China 3.Henan Univ, Coll Life Sci, Int Joint Res Lab Global Change Ecol, Kaifeng 475004, Henan, Peoples R China 4.Deakin Univ, Sch Life & Environm Sci, Ctr Chem & Biotechnol, Geelong, Vic 3200, Australia
推荐引用方式 GB/T 7714	Guo, Meixia,Gong, Zongqiang,Miao, Renhui,et al. Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: Bacterial community and functional gene expression mechanisms[J]. CHEMOSPHERE,2018,212:15-23.
APA	Guo, Meixia.,Gong, Zongqiang.,Miao, Renhui.,Jia, Chunyun.,Rookes, James.,...&Zhuang, Jie.(2018).Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: Bacterial community and functional gene expression mechanisms.CHEMOSPHERE,212,15-23.
MLA	Guo, Meixia,et al."Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: Bacterial community and functional gene expression mechanisms".CHEMOSPHERE 212(2018):15-23.