Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils

doi:10.1016/j.soilbio.2015.08.040

	Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils
	Bai, Ren 1,2; Xi, Dan 1,3; He, Ji-Zheng 2,4; Hu, Hang-Wei 4; Fang, Yun-Ting 3; Zhang, Li-Mei 2
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2015-12-01
卷号	91 页码:212-221
关键词	Anammox Hzs Gene Rice Paddy Soil Soil Profile N-15 Tracer Denitrification
ISSN号	0038-0717
DOI	10.1016/j.soilbio.2015.08.040
英文摘要	Anaerobic ammonium oxidation (anammox) is a globally important nitrogen-cycling process mediated by specialized microbes, and has been demonstrated to be ubiquitous in anoxic natural settings and bioreactors. However, our knowledge of its prevalence in different paddy soil types and along the depth profiles remains largely undocumented. Here, mesocosm incubations were constructed to investigate the activity and community compositions of anammox bacteria across different depth layers of three different paddy soils. N-15 tracer experiments showed that anathmox rates were prevalent in almost all depth layers of an inceptisol (Binhai, BH) and an oxisol (Leizhou, LZ), but was absent in an ultisol (Taoyuan, TY). The functional gene hzsB of anammox bacteria was only detected in BH and 12 soil profiles with detected anammox activity. Anammox accounted for 0.4%-12.2% of the total N-2 production, and anammox rates were 0.02-0.77 nmol N g(-1) dry soil h-(1) and significantly correlated with soil pH and electric conductivity. Anammox activity and abundance of hzsB genes were significantly lower in the surface soil layers (0-5 cm) than in the sub-surface soil layers (20-60 cm). Candidatus Brocadia, Candidatus Kuenenia and Candidatus Jettenia were detected by cloning and sequencing, with Candidatus Brocadia being dominant in all the three soils and Candidatus Jettenia being more frequently detected in the 12 soil. The operational taxonomic unit number and diversity indices of anammox bacterial 16S rRNA gene increased with soil depth. Our results demonstrate that anammox is more common and active in alkaline soils than in acidic soils, in deep paddy soil layers than in surface soil layers, and that anammox activities are likely to be regulated by soil chemical properties such as pH, salinity and redox potential. (C) 2015 Elsevier Ltd. All rights reserved.
WOS研究方向	Agriculture
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000364502900024
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122261]
专题	中国科学院沈阳应用生态研究所
通讯作者	Zhang, Li-Mei
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110164, Peoples R China 4.Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic 3010, Australia
推荐引用方式 GB/T 7714	Bai, Ren,Xi, Dan,He, Ji-Zheng,et al. Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils[J]. SOIL BIOLOGY & BIOCHEMISTRY,2015,91:212-221.
APA	Bai, Ren,Xi, Dan,He, Ji-Zheng,Hu, Hang-Wei,Fang, Yun-Ting,&Zhang, Li-Mei.(2015).Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils.SOIL BIOLOGY & BIOCHEMISTRY,91,212-221.
MLA	Bai, Ren,et al."Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils".SOIL BIOLOGY & BIOCHEMISTRY 91(2015):212-221.