Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots

doi:10.1038/srep15127

	Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots
	Ma, Keping 5; Pei, Nancai 1,2; Erickson, David L.3; Chen, Bufeng 1,2; Ge, Xuejun 4; Mi, Xiangcheng 5; Swenson, Nathan G.6; Zhang, Jin-Long 7; Jones, Frank A.8; Huang, Chun-Lin 9
刊名	SCIENTIFIC REPORTS
	2015-10-12
卷号	5
ISSN号	2045-2322
DOI	10.1038/srep15127
英文摘要	To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots (FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone) and compared the rates of species discrimination (RSD). We quantified RSD by two DNA barcode combinations (rbcL + matK and rbcL + matK + trnH-psbA) using a monophyly-based method (GARLI). We defined two indexes of closely-related taxa (G(m)/G(t) and S/G ratios) and correlated these ratios with RSD. The combination of rbcL + matK averagely discriminated 88.65%, 83.84% and 72.51% at the local, regional and global scales, respectively. An additional locus trnH-psbA increased RSD by 2.87%, 1.49% and 3.58% correspondingly. RSD varied along a latitudinal gradient and were negatively correlated with ratios of closely-related taxa. Successes of species discrimination generally depend on scales in global FDPs. We suggested that the combination of rbcL + matK + trnH-psbA is currently applicable for DNA barcoding-based phylogenetic studies on forest communities.
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000362563500001
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122227]
专题	中国科学院沈阳应用生态研究所
通讯作者	Pei, Nancai
作者单位	1.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 2.Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China 3.Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, Washington, DC 20013 USA 4.Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China 5.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 6.Univ Maryland, Dept Biol, College Pk, MD 20742 USA 7.Kadoorie Farm & Bot Garden, Flora Conservat Dept, Tai Po, Hong Kong, Peoples R China 8.Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA 9.Natl Museum Nat Sci, Dept Biol, Lab Mol Phylogenet, Taichung, Taiwan 10.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Ma, Keping,Pei, Nancai,Erickson, David L.,et al. Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots[J]. SCIENTIFIC REPORTS,2015,5.
APA	Ma, Keping.,Pei, Nancai.,Erickson, David L..,Chen, Bufeng.,Ge, Xuejun.,...&Kress, W. John.(2015).Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots.SCIENTIFIC REPORTS,5.
MLA	Ma, Keping,et al."Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots".SCIENTIFIC REPORTS 5(2015).