Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles

doi:10.1021/acs.est.5b02102

	Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles
	An, Jing 2; Hu, Xiangang 3; Ouyang, Shaohu 3; Mu, Li 1; Zhou, Qaing 3
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2015-09-15
卷号	49 期号:18 页码:10825-10833
ISSN号	0013-936X
DOI	10.1021/acs.est.5b02102
英文摘要	Nanomaterial oxides are common formations of nanomaterials in the natural environment. Herein, the nanotoxicology of typical graphene oxide (GO) and carboxyl single-walled carbon nanotubes (C-SWCNT) was compared. The results showed that cell division of Chlorella vulgaris was promoted at 24 h and then inhibited at 96 h after nanomaterial exposure. At 96 h, GO and C-SWCNT inhibited the rates of cell division by 0.08-15% and 0.8-28.3%, respectively. Both GO and C-SWCNT covered the cell surface, but the uptake percentage of C-SWCNT was 2-fold higher than that of GO. C-SWCNT induced stronger plasmolysis and mitochondrial membrane potential loss and decreased the cell viability to a greater extent than GO. Moreover, C-SWCNT-exposed cells exhibited more starch grains and lysosome formation and higher reactive oxygen species (ROS) levels than GO-exposed cells. Metabolomics analysis revealed significant differences in the metabolic profiles among the control, C-SWCNT and GO groups. The metabolisms of alkanes, lysine, octadecadienoic acid and valine was associated with ROS and could be considered as new biomarkers of ROS. The nanotoxicological mechanisms involved the inhibition of fatty acid, amino acid and small molecule acid metabolisms. These findings provide new insights into the effects of GO and C-SWCNT on cellular responses.
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000361415800009
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122195]
专题	中国科学院沈阳应用生态研究所
通讯作者	Zhou, Qaing
作者单位	1.Minist Agr, Inst Agroenvironm Protect, Tianjin 300191, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China 3.Nankai Univ, Coll Environm Sci & Engn, Tianjin Key Lab Environm Remediat & Pollut Contro, Minist Educ,Key Lab Pollut Proc & Environm Criter, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	An, Jing,Hu, Xiangang,Ouyang, Shaohu,et al. Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2015,49(18):10825-10833.
APA	An, Jing,Hu, Xiangang,Ouyang, Shaohu,Mu, Li,&Zhou, Qaing.(2015).Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles.ENVIRONMENTAL SCIENCE & TECHNOLOGY,49(18),10825-10833.
MLA	An, Jing,et al."Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles".ENVIRONMENTAL SCIENCE & TECHNOLOGY 49.18(2015):10825-10833.