Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure

doi:10.1016/j.snb.2018.03.039

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	Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure
	Zeng, Yan1,2 ; Zhang, Dun 1,2; Qi, Peng1,2 ; Zheng, Laibao1,2,3
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-07-15
卷号	265 页码:468-475
关键词	Label free DNA junction G-quadruplex DNA DNA detection Lysozyme detection
ISSN号	0925-4005
DOI	10.1016/j.snb.2018.03.039
通讯作者	Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
英文摘要	Label free analysis of multiplex analytes is of great demand in modern bio-analytical science. In this study, a novel DNA junction coupled with two different G-quadruplex DNA structures was demonstrated, achieving colorimetric and fluorescent signal report. Upon the introduction of different targets (including DNA and Lysozyme), the specific interaction between targets and sensing DNA junction could induce the reaction of exonuclease-stimulated recycling of target DNA and conformation transformation based on aptamer/lysozyme binding. With the integration of G-quadruplex/Hemin as colorimetric probe and G-quadruplex/PPIX as fluorescence probe, this label-free sensing system showed high sensitivity and selectivity for DNA and lysozyme detection. Furthermore, we also demonstrated that this novel sensing method could respond to these two targets independently and simultaneously, promising a great hope for its potential application in analytical fields. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; China Postdoctoral Science Foundation[2016 M602199] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-ES02]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000430232500057
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158938]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Zhang, Dun; Qi, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,265:468-475.
APA	Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2018).Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure.SENSORS AND ACTUATORS B-CHEMICAL,265,468-475.
MLA	Zeng, Yan,et al."Label-free simultaneous sensor for multi-components detection based on G-Quadruplex DNA structure".SENSORS AND ACTUATORS B-CHEMICAL 265(2018):468-475.