Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation

doi:10.3390/md17010050

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation
	Wang, Ju 1,2,3; Liu, Ge 1,2,4; Ma, Weiping 5; Lu, Zhongxia 5; Sun, Chaomin 1,2,4
刊名	MARINE DRUGS
	2019
卷号	17 期号:1 页码:15
关键词	polysaccharide EPS11 cancer filiform structure adhesion CD99 metastasis
ISSN号	1660-3397
DOI	10.3390/md17010050
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要	Our previous results suggested that EPS11, a novel marine bacterial polysaccharide, might be a potential drug candidate for human non-small cell lung carcinoma treatment. In this study, we further investigate the anticancer mechanisms against liver cancer and the anti-metastatic effects in vivo of EPS11. Firstly, we found that EPS11 exerts cytotoxic effects via blocking cell adhesion and destroying filiform structure formation in Huh7.5 cells. Moreover, mass spectrometry-based proteomic analysis of EPS11-treated Huh7.5 cells revealed that expression of many adhesion-related proteins was significantly changed. It is noteworthy that the expression of CD99, a key factor related to cell adhesion, migration and cell death, is remarkably down-regulated after EPS11 treatment. Importantly, over-expression of CD99 partly rescues cell death rate, and improves cell adhesion and migration ability in Huh7.5 treated by EPS11. Thus, we propose that CD99 is a potential action target of EPS11, inhibiting cancer cell proliferation, adhesion and migration. Notably, administration of EPS11 simultaneously with tumor induction evidently reduces tumor nodule formation in the lungs, which strongly indicates that EPS11 has anti-metastatic effects in vivo. Taken together, our results suggest that EPS11 inhibits liver cancer cell growth via blocking cell adhesion and attenuating filiform structure formation, and has potential as an anti-cancer drug, targeting metastasis of cancer cells, in the future.
资助项目	National Key R&D Program of China[2018YFC0310800] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; Tai Shan Young Scholar Foundation of Shandong Province[tsqn20161051] ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; 100-Talent Project of the Chinese Academy of Sciences
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000458053200050
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/160858]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ju,Liu, Ge,Ma, Weiping,et al. Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation[J]. MARINE DRUGS,2019,17(1):15.
APA	Wang, Ju,Liu, Ge,Ma, Weiping,Lu, Zhongxia,&Sun, Chaomin.(2019).Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation.MARINE DRUGS,17(1),15.
MLA	Wang, Ju,et al."Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation".MARINE DRUGS 17.1(2019):15.