Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection

doi:10.1111/are.13637

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection
	Du, Yishuai 1; Liu, Pengfei 1,2; Meng, Lingjie1,2 ; Sharawy, Zaki 3; Liu, Ying4
刊名	AQUACULTURE RESEARCH
	2018-05-01
卷号	49 期号:5 页码:1826-1833
关键词	Aeromonassalmonicida Atlantic salmon colonization immersion infection route
ISSN号	1355-557X
DOI	10.1111/are.13637
通讯作者	Liu, Ying(yingliu@dlou.edu.cn)
英文摘要	Aeromonassalmonicida subsp. masoucida (ASM) is classified as atypical A.salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS-C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salarL.) by an immersion challenge with the control group (T0, no AS-C4), group T1 (2.67x10(4) CFU/ml AS-C4) and group T2 (2.67x10(7) CFU/ml AS-C4). The numbers of AS-C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real-time PCR (qRT-PCR). AS-C4 were detected in the gill and intestine as early as 0hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS-C4 into salmon. Although AS-C4 could not be detected in the skin until 24hr after the challenge in T1 group, it could be detected in the skin as early as 0hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS-C4 into salmon. AS-C4 was immediately detected in the blood within 3hr after it entered the host, suggesting that AS-C4 successfully invaded the bloodstream of fish. After AS-C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture.
资助项目	National Key R&D Program of China[2017YFD0701700] ; 56th China Postdoctoral Science Foundation[2014M560580] ; Postdoctoral Innovation Project Special Funds of Shandong Province[201402005] ; Qingdao Innovation Talents Program[13-CX-16]
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000429410800009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158671]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Ying
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing, Peoples R China 3.Natl Inst Oceanog & Fisheries NIOF, Aquaculture Div, Suez, Egypt 4.Dalian Ocean Univ, Marine Sci & Environm Engn Sch, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Du, Yishuai,Liu, Pengfei,Meng, Lingjie,et al. Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection[J]. AQUACULTURE RESEARCH,2018,49(5):1826-1833.
APA	Du, Yishuai,Liu, Pengfei,Meng, Lingjie,Sharawy, Zaki,&Liu, Ying.(2018).Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection.AQUACULTURE RESEARCH,49(5),1826-1833.
MLA	Du, Yishuai,et al."Colonization of Aeromonassalmonicida subsp masoucida strains in Atlantic salmon (Salmo salar L.) during infection".AQUACULTURE RESEARCH 49.5(2018):1826-1833.