A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas

doi:10.1016/j.dci.2017.12.029

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas
	Jia, Yunke 1,3; Yang, Bin 1,3; Dong, Wenjing1,3 ; Liu, Zhaoqun4 ; Lv, Zhao 1,3; Jia, Zhihao 1,3; Qiu, Limei 1; Wang, Lingling 2,4; Song, Linsheng 2,4
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2018-05-01
卷号	82 页码:83-93
关键词	Serotonin Serotonin receptor 1A Cellular immune response Humoral immune response Crassostrea gigas
ISSN号	0145-305X
DOI	10.1016/j.dci.2017.12.029
通讯作者	Wang, Lingling(wanglingling@dlou.edu.cn) ; Song, Linsheng()
英文摘要	Serotonin receptors, including ligand-gated ion channel (LGICs) and G protein-coupled receptors (GPCR), play vital roles in modulating physiological processes and immunoreaction. In the present study, a homologue of serotonin (5-HT) receptor was identified from oyster Crassostrea gigas (designated Cg5-HTR1). Its open reading frame (ORF) was of 1239 bp, encoding-a polypeptide of 412 amino acids with a seven transmembrane region. Cg5-HTR-1 shared high similarity with the 5-HTRs from other animals. The cAMP contents in HEK293T cells decreased significantly after Cg5-HTR-1 transfection and 5-HT incubation (p < .05), while blocking Cg5-HTR-1 with specific receptor antagonist reversed this downtrend. The intracellular Ca2+ concentrations increased significantly (p < .05) after cell transfection and 5-HT incubation, and the antagonist treatment also arrested this process. Cg5-HTR-1 transcripts were widely distributed in various tissues, with the highest level in hepatopancreas and lowest level in mantle and gill. The mRNA expression of Cg5-HTR-1 in hemocyte increased significantly after lipopolysaccharide (LPS) stimulation and reached the peak level (6.47-fold, p < .05) at 6 h post treatment. The inhibition of Cg5-HTR-1 significantly reduced the expression of tumor necrosis factor (TNF) mRNA in hemocyte, down-regulated the superoxide dismutase (SOD) activity in serum, and induced the apoptosis of hemocyte (p < .05). These results suggested that Cg5-HTR-1 was a novel member of 5-HT1 receptor family and it mediated serotonergic immunomodulation on both cellular and humoral immune responses. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	National Science Foundation of China[U1706204] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Aoshan Talent Cultivation Program
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000427668700009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158366]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Lingling; Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Jia, Yunke,Yang, Bin,Dong, Wenjing,et al. A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:83-93.
APA	Jia, Yunke.,Yang, Bin.,Dong, Wenjing.,Liu, Zhaoqun.,Lv, Zhao.,...&Song, Linsheng.(2018).A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,83-93.
MLA	Jia, Yunke,et al."A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):83-93.