Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis

doi:10.1016/j.gene.2018.01.043

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis
	Li, Shihao 1,2; Li, Fuhua 1,2; Yu, Kuijie 1; Xiang, Jianhai1
刊名	GENE
	2018-04-05
卷号	649 页码:1-7
关键词	Doublesex Insulin-like androgenic gland hormone Expression Sex determination Shrimp
ISSN号	0378-1119
DOI	10.1016/j.gene.2018.01.043
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	The doublesex and its homologue genes are important regulators of sexual differentiation which are conserved among animal kingdom. In the present study, we reported a doublesex gene (designated as FcDsx) identified from the Chinese shrimp F. chinensis. The gene structure, nucleotide and deduced amino acid sequences of FcDsx were characterized. The results showed that the deduced amino acid sequence of FcDsx had the common features of Dsx proteins, including a doublesex/male abnormal 3 (DM) domain, an oligomerization domain and a predicted monopartite nuclear localization signal. The expression patterns of FcDsx in different tissues and developmental stages were detected. FcDsx exhibited a sex-biased expression patterns in different tissues and its expression level increased along with developmental stages. In addition, its regulation on the expression of FcIAG, a gene important for sexual differentiation of male crustacean, was also analyzed. Putative Dsx binding site was identified on the promoter region of FcIAG and knockdown of FcDsx could reduce the expression of FcIAG, which suggested that FcDsx might be the upstream regulator of FcIAG. The present data indicated that FcDsx gene might involve in shrimp sexual differentiation process.
资助项目	National Natural Science Foundation of China[31302171] ; National Natural Science Foundation of China[31461143007] ; China Agriculture Research system-48[CARS-48] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000427314600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158145]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Shihao,Li, Fuhua,Yu, Kuijie,et al. Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis[J]. GENE,2018,649:1-7.
APA	Li, Shihao,Li, Fuhua,Yu, Kuijie,&Xiang, Jianhai.(2018).Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis.GENE,649,1-7.
MLA	Li, Shihao,et al."Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis".GENE 649(2018):1-7.