Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer

doi:10.3964/j.issn.1000-0593(2017)07-2110-05

	Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer
	Huang Y(黄艳); Tao Y(陶冶); Liu ZT(刘志桐); Liu, ZT; Gao, ZH; Sun DR(孙大睿); Tao, Y; Sun, DR; Huang, Y; Gao ZH(高振华)
刊名	SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析
	2017
卷号	37 期号:7 页码:2110-2114
关键词	Microfluidic mixing Continuous flow Synchrotron radiation circular dichroism Protein folding
ISSN号	1000-0593
DOI	10.3964/j.issn.1000-0593(2017)07-2110-05
其他题名	基于微流控混合器的连续流动态同步辐射圆二色谱发展
文献子类	Article
英文摘要	The setup of dynamic synchrotron radiation dichroism spectroscopy (SRCD) is discussed by using continuous flow probe with microfluidic mixer, developed at 4B8 ultraviolet vacuum spectroscopy beamline at Beijing Synchrotron Radiation Facility. The quartz microfluidic mixer was fabricated with deep ion etching with channel depth down to 44. 5 microns. The mixer is designed on serpentine configuration to reach the effective mixing. The mixing efficiency was evaluated based on fluorescence image of the mixing solution under practical high viscous solution condition. Dynamic SRCD measurement with mixer was validated after implementing focus position feedback, since wavelength dispersion of the focusing lens results in the changes of focus spot position during wavelength scan. The time range covers from 4. 5 to 270 ms at present under 500 (L . min' flow rate within the wide observation channel. The performance of the,time-resolved SRCD is demonstrated in snapshoting the folding of cytochrome c back to 54% at 4. 5 millisecond after completely mixing.
WOS研究方向	Spectroscopy
语种	中文
WOS记录号	WOS:000405648400023
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285234]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Huang Y,Tao Y,Liu ZT,et al. Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析,2017,37(7):2110-2114.
APA	黄艳.,陶冶.,刘志桐.,Liu, ZT.,Gao, ZH.,...&汪皓.(2017).Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer.SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析,37(7),2110-2114.
MLA	黄艳,et al."Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer".SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析 37.7(2017):2110-2114.