Biosafety study and mechanism comparison on two types of silica with different nanostructures

doi:10.1039/c7tx00076f

	Biosafety study and mechanism comparison on two types of silica with different nanostructures
	Xing, GM; He, B; Dai, WB; Zhang, H; Yuan, L; Wu, HN; Zhao, B; Chen, XH; Zhang, Y; Xing GM(邢更妹)
刊名	TOXICOLOGY RESEARCH
	2017
卷号	6 期号:4 页码:487-498
ISSN号	2045-452X
DOI	10.1039/c7tx00076f
文献子类	Article
英文摘要	Silica is frequently used in oral drug delivery; however, its biosafety, particularly concerned with its nanostructure, has not been comprehensively studied yet. Here, the in vitro and in vivo biosafety of two types of silica (A200, nano-sized or micron-sized agglomerates; S350, micro-sized particles with nanopores) were compared and the possible reasons for the differences were explored. The results indicated that both A200 and S350 could inhibit the growth of Caco-2 cells by inducing apoptosis and changing the cell cycle progression. A200 showed a stronger influence than S350 in most of the in vitro experiments. In the in vivo study in KM mice, both A200 and S350 could change the blood constituents under the tested conditions; A200 also increased the levels of inflammatory factors in plasma and the numbers of CD4+ lymphocyte subsets. No obvious organic damage was observed in either the A200-treated or S350-treated groups. The transport study showed that neither A200 nor S350 were readily transported across the intestinal epithelial barrier in vitro and in vivo, but A200 could transport across the lymphaticassociated epithelium and accumulate in the Peyer's Patches, which might explain the A200-induced immune response. The increased transport of A200 might relate to its particle size, dispersion state and specific surface area. In conclusion, these results demonstrated that A200 and S350 exhibited diverse biosafety aspects, which correlated with their different nanostructures. We believe this study will provide some scientific information about the biosafety of A200 and S350 for their applications in oral drug delivery systems.
电子版国际标准刊号	2045-4538
WOS关键词	IN-VIVO BIODISTRIBUTION ; OXIDATIVE STRESS ; VITRO TOXICITY ; PARTICLE-SIZE ; NANOPARTICLES ; CELLS ; DELIVERY ; CYTOTOXICITY ; ASSAY ; PHARMACOKINETICS
WOS研究方向	Toxicology
语种	英语
WOS记录号	WOS:000404873500011
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285230]
专题	高能物理研究所_多学科研究中心高能物理研究所_粒子天体物理中心中国科学院高能物理研究所_中国散裂中子源
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Xing, GM,He, B,Dai, WB,et al. Biosafety study and mechanism comparison on two types of silica with different nanostructures[J]. TOXICOLOGY RESEARCH,2017,6(4):487-498.
APA	Xing, GM.,He, B.,Dai, WB.,Zhang, H.,Yuan, L.,...&Zhang, Q.(2017).Biosafety study and mechanism comparison on two types of silica with different nanostructures.TOXICOLOGY RESEARCH,6(4),487-498.
MLA	Xing, GM,et al."Biosafety study and mechanism comparison on two types of silica with different nanostructures".TOXICOLOGY RESEARCH 6.4(2017):487-498.