Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination

doi:10.1002/smll.201601600

	Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination
	Shu, D; Montemagno, C; Ding, BQ; Li, JG; Guo, PX; Li JY(李敬源); Zhang, H; Zhao, Z
刊名	SMALL
	2017
卷号	13 期号:1 页码:1601600
ISSN号	1613-6810
DOI	10.1002/smll.201601600
文献子类	Article
英文摘要	The significance of bionanomotors in nanotechnology is analogous to mechanical motors in daily life. Here the principle and approach for designing and constructing biomimetic nanomotors with continuous single-directional motion are reported. This bionanomotor is composed of a dodecameric protein channel, a six-pRNA ring, and an ATPase hexamer. Based on recent elucidations of the one-way revolving mechanisms of the phi29 double-stranded DNA (dsDNA) motor, various RNA and protein elements are designed and tested by single-molecule imaging and biochemical assays, with which the motor with active components has been constructed. The motor motion direction is controlled by three operation elements: (1) Asymmetrical ATPase with ATP-interacting domains for alternative DNA binding/pushing regulated by an arginine finger in a sequential action manner. The arginine finger bridges two adjacent ATPase subunits into a non-covalent dimer, resulting in an asymmetrical hexameric complex containing one dimer and four monomers. (2) The dsDNA translocation channel as a one-way valve. (3) The hexameric pRNA ring geared with left-/right-handed loops. Assessments of these constructs reveal that one inactive subunit of pRNA/ATPase is sufficient to completely block motor function (defined as K = 1), implying that these components work sequentially based on the principle of binomial distribution and Yang Hui's triangle.
电子版国际标准刊号	1613-6829
WOS关键词	DNA-PACKAGING MOTOR ; WAY VALVE MECHANISM ; ARGININE FINGER ; BACTERIOPHAGE-PHI-29 DNA ; BACILLUS-SUBTILIS ; ATPASE ACTIVITY ; NUCLEIC-ACIDS ; AAA+ PROTEINS ; PHAGE PHI-29 ; GENOMIC DNA
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000396562400004
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285183]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Shu, D,Montemagno, C,Ding, BQ,et al. Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination[J]. SMALL,2017,13(1):1601600.
APA	Shu, D.,Montemagno, C.,Ding, BQ.,Li, JG.,Guo, PX.,...&Zhao, Z.(2017).Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination.SMALL,13(1),1601600.
MLA	Shu, D,et al."Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination".SMALL 13.1(2017):1601600.