Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application

doi:10.1007/s00216-017-0606-1

	Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application
	Liu B(刘冰); Xu BG(徐炳刚); Dong JQ(董金泉); Sun BY(孙宝云); Guo, Xihong; Yang, Shangyuan; Huang, Huan; Cui, Rongli; Li, Cheng; Yao, Huanli
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2017
卷号	409 期号:28 页码:6575-6581
关键词	Metallofullerene Enzyme immunoassay Polyclonal antibody Immunogen Nanomedicine
ISSN号	1618-2642
DOI	10.1007/s00216-017-0606-1
文献子类	Article
英文摘要	With their intriguing structures and properties, metallofullerenols have attracted considerable attention in biological and medical applications. Due to the increasing biomedical interest, effective detection methods are important to monitor and control metallofullerenols. However, the detection of metallofullerenols becomes very difficult after polyhydroxylated modification due to the lack of detectable features. Antibody-based immunoassay methods have been important tools for detection and will better meet the needs of analysis of metallofullerenols. Thus, the antigenicity of metallofullerenol has been studied for the first time. In this study, no immune response was detected when metallofullerenol Gd@C-82(OH)(x) was used as immunogen. However, the polyclonal antibody against metallofullerenol was produced using metallofullerenol-KLH (keyhole limpet hemocyanin) as immunogen, indicating that metallofullerenol can act as hapten. The specificity of the obtained antibody was investigated. It has been found that the hydroxyl groups on the surface of the carbon cage, the encapsulated metal, and the size and shape of the carbon cage did not affect the recognition specificity of the antibody. Based on the obtained antibody, an indirect competitive enzyme immunoassay was developed for the determination of metallofullerenol with detection limits of 18 ng/mL in PBS. This enzyme immunoassay method was successfully used to detect metallofullerenol in serum. This work can provide an innovative way to determine metallofullerenols.
电子版国际标准刊号	1618-2650
WOS关键词	MRI CONTRAST AGENTS ; MONOCLONAL-ANTIBODIES ; NANOPARTICLES ; FULLERENES ; RELAXIVITY ; WATER ; C-60
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
WOS记录号	WOS:000414329900006
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/284995]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Liu B,Xu BG,Dong JQ,et al. Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(28):6575-6581.
APA	刘冰.,徐炳刚.,董金泉.,孙宝云.,Guo, Xihong.,...&张乐乐.(2017).Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(28),6575-6581.
MLA	刘冰,et al."Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.28(2017):6575-6581.