Glutaredoxin 1 mediates the protective effect of steady laminar flow on endothelial cells against oxidative stress-induced apoptosis via inhibiting Bim

doi:10.1038/s41598-017-15672-3

	Glutaredoxin 1 mediates the protective effect of steady laminar flow on endothelial cells against oxidative stress-induced apoptosis via inhibiting Bim
	Wang, Jing; Hu, Yi; Yan, Chen; Li, Yao; Ren, Meng; Wang, Xiaoqun; Cui, Xingxing; Zhao, Hongmei; Zhao, Chuanrong; Zhou, Jing
刊名	SCIENTIFIC REPORTS
	2017
卷号	7 页码:15539
ISSN号	2045-2322
DOI	10.1038/s41598-017-15672-3
文献子类	Article
英文摘要	Endothelial cell apoptosis induced by oxidative stress is an early event in the development of atherosclerosis. Several antioxidant enzymes which can cope with oxidative stress are up-regulated by the anti-atherogenic laminar blood flow often seen in straight or unbranched regions of blood vessels. However, the molecular mechanism responsible for flow-induced beneficial effects is incompletely understood. Here we report the role of glutaredoxin 1 (Grx1), an antioxidant enzyme, in flow-mediated protective effect in endothelial cells. Specifically, we found that Grx1 is markedly up-regulated by the steady laminar flow. Increasing Grx1 reduces the pro-apoptotic protein Bim expression through regulating Akt-FoxO1 signaling and also attenuates H2O2-induced Bim activation via inhibiting JNK phosphorylation, subsequently preventing the apoptosis of endothelial cells. Grx1 knockdown abolishes the inhibitory effect of steady laminar flow on Bim. The inhibitory effect of Grx1 on Bim is dependent on Grx1's thioltransferase activity. These findings indicate that Grx1 induction plays a key role in mediating the protective effect of laminar blood flow and suggest that Grx1 may be a potential therapeutic target for atherosclerosis.
WOS关键词	BCL-2 PROTEIN FAMILY ; RED-BLOOD-CELLS ; BAX ACTIVATION ; UV-IRRADIATION ; DEATH PATHWAY ; AKT ; JNK ; GLUTATHIONYLATION ; PHOSPHORYLATION ; IDENTIFICATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000415166000035
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/284867]
专题	高能物理研究所_多学科研究中心高能物理研究所_加速器中心中国科学院高能物理研究所_中国散裂中子源
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Wang, Jing,Hu, Yi,Yan, Chen,et al. Glutaredoxin 1 mediates the protective effect of steady laminar flow on endothelial cells against oxidative stress-induced apoptosis via inhibiting Bim[J]. SCIENTIFIC REPORTS,2017,7:15539.
APA	Wang, Jing.,Hu, Yi.,Yan, Chen.,Li, Yao.,Ren, Meng.,...&Berk, Bradford.(2017).Glutaredoxin 1 mediates the protective effect of steady laminar flow on endothelial cells against oxidative stress-induced apoptosis via inhibiting Bim.SCIENTIFIC REPORTS,7,15539.
MLA	Wang, Jing,et al."Glutaredoxin 1 mediates the protective effect of steady laminar flow on endothelial cells against oxidative stress-induced apoptosis via inhibiting Bim".SCIENTIFIC REPORTS 7(2017):15539.