A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood

doi:10.1007/s00216-019-01738-w

	A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood
	Gao, Tingjuan 5,6; Tang, Yuchen 5,6; Smith, Zachary J.1; Chen, Yangfei 5,6; Wang, Bin 2; Fan, Pengwei 2; Lei, Youwang 3; Liu, Hongping 4; Deng, Qiong 5,6; Li, Xinyi 5,6
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019-05-01
卷号	411 期号:13 页码:2767-2780
关键词	Blood counting POCT Image-based cytometry Sample preparation
ISSN号	1618-2642
DOI	10.1007/s00216-019-01738-w
英文摘要	Blood counting is one of the most commonly ordered clinical assays, and is often part of the basis for initial diagnosis and screening for disease. While substantial prior research has shown the ability of portable instruments to accurately produce blood counts through image- or flow-based cytometry, these methods require complex sample preparation using either costly commercial imaging chambers or complicated reagents. To address these issues, in this paper we developed a method to prepare trace volumes of whole blood aimed at portable blood counting. The strategy is based on pre-storing dry-form reagents and fabricating a specifically designed cell counter. In order to obtain total cell counts for red blood cells, platelets, and 3-part differentials of white blood cells, two parallel counting chambers with different depths are made from cost- and environmentally friendly materials using soft lithography. As little as 1l of whole blood is prepared with pre-stored reagents in centrifuge vials, whereas red blood cells are sphered and white blood cells are stained at the same time. Driven by the capillary force, prepared blood samples enter the hydrophilic chambers automatically. Monolayers of cells are formed when the blood dilution factors and the chamber depths are co-optimized. Combined with our previous custom-built instrument and automated analysis algorithm, the sample preparation strategy allows producing counting results with excellent agreement to a gold-standard clinical hematology instrument. The success of this preparation method may further advance applications of our technology for global use in low-resource settings where central hematology laboratories are not accessible.
资助项目	National Natural Science Foundation of China[21605054] ; Central China Normal University (CCNU)[210-31102] ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE)[234-20205016002] ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE)[234-20205170355] ; Program of Introducing Talents of Discipline to University of China (111 program)[B17019] ; CCNU Program of Innovation and Entrepreneurship for college students[220-20205180419] ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China[2016YFA020130]
WOS关键词	DYE ; COMPACT
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000468133600004
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Central China Normal University (CCNU) ; Central China Normal University (CCNU) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; CCNU from the colleges' basic research and operation of Ministry of Education (MOE) ; Program of Introducing Talents of Discipline to University of China (111 program) ; Program of Introducing Talents of Discipline to University of China (111 program) ; CCNU Program of Innovation and Entrepreneurship for college students ; CCNU Program of Innovation and Entrepreneurship for college students ; 1000 Young Talents Global Recruitment Plan ; 1000 Young Talents Global Recruitment Plan ; Ministry of Science and Technology of the People's Republic of China ; Ministry of Science and Technology of the People's Republic of China
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13635]
专题	中国科学院武汉物理与数学研究所
通讯作者	Gao, Tingjuan; Tang, Yuchen
作者单位	1.Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, Hefei 230027, Anhui, Peoples R China 2.Wuhan 7 Hosp, Clin Lab Ctr, Wuhan 430071, Hubei, Peoples R China 3.Jieyang Women & Childrens Hlth Care Hosp, Prenatal Diagnost Ctr, Jieyang 522000, Guangdong, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 5.Cent China Normal Univ, Coll Chem, Chem Biol Ctr, Wuhan 430079, Hubei, Peoples R China 6.Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Gao, Tingjuan,Tang, Yuchen,Smith, Zachary J.,et al. A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(13):2767-2780.
APA	Gao, Tingjuan.,Tang, Yuchen.,Smith, Zachary J..,Chen, Yangfei.,Wang, Bin.,...&Li, Xinyi.(2019).A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(13),2767-2780.
MLA	Gao, Tingjuan,et al."A smart preparation strategy for point-of-care cellular counting of trace volumes of human blood".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.13(2019):2767-2780.