Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)

doi:10.1111/grs.12222

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国西南野生生物种质资源库

	Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)
	Li, Wenjun 1; Su, Zhihao 1; Li, Airong2 ; Guan, Kaiyun 1,2; Feng, Ying 1
刊名	GRASSLAND SCIENCE
	2019-04-01
卷号	65 期号:2 页码:135-138
关键词	invasive native species Pedicularis kansuensis polymorphic microsatellites
ISSN号	1744-6961
DOI	10.1111/grs.12222
通讯作者	Feng, Ying(luckfy@ms.xjb.ac.cn)
英文摘要	To quantify the population-level genetic characteristics of Pedicularis kansuensis, polymorphic microsatellite loci (simple sequence repeats) were identified and characterized. Methods and Results: Using Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified 1594 sequences by four softwares simultaneously. We developed and characterized 18 polymorphic microsatellite primers for P. kansuensis using 45 individuals that were collected from three P. kansuensis populations. The number of alleles varied from two to 12. The observed heterozygosity and expected heterozygosity per locus ranged from 0.182 to 0.933 and 0.227 to 0.904 in the pooled population, respectively. All of the primers that were developed were also successfully applied in Pedicularis cheilanthifolia and Pedicularis violascens. These markers should be useful in probing the genetic diversity, genetic structure of P. kansuensis, which could provide information about assessment and prediction of potential risks of this species in its distribution areas.
WOS研究方向	Agriculture
语种	英语
WOS记录号	WOS:000462884700008
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/67682]
专题	昆明植物研究所_中国西南野生生物种质资源库
通讯作者	Feng, Ying
作者单位	1.Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land, Xinjiang Inst Ecol & Geog, Urumqi, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Res & Dev Wild Plant Resources, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenjun,Su, Zhihao,Li, Airong,et al. Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)[J]. GRASSLAND SCIENCE,2019,65(2):135-138.
APA	Li, Wenjun,Su, Zhihao,Li, Airong,Guan, Kaiyun,&Feng, Ying.(2019).Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae).GRASSLAND SCIENCE,65(2),135-138.
MLA	Li, Wenjun,et al."Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)".GRASSLAND SCIENCE 65.2(2019):135-138.