Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass

doi:10.1021/acs.jafc.9b00027

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	Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass
	Zhang, Rui 1,2; Li, Na 1,2; Xu, Shujing 1,2; Han, Xiaowei 1,2; Li, Chunyan 1,2; Wei, Xin 3; Liu, Yu 1,2; Tu, Tao 4; Tang, Xianghua 1,2; Zhou, Junpei 1,2
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2019-03-20
卷号	67 期号:11 页码:3220-3228
关键词	beta-xylosidase beta-1,2-glycosidic bond enzyme commission notoginsenosides glycoside hydrolase family 39
ISSN号	0021-8561
DOI	10.1021/acs.jafc.9b00027
通讯作者	Zhou, Junpei(junpeizhou@ynnu.edu.cn) ; Huang, Zunxi(huangzunxi@163.com)
英文摘要	beta-1,2-Xylosidase activity has not been recorded as an EC subsubclass. In this study, phylogenetic analysis and multiple sequence alignments revealed that characterized beta-xylosidases of glycoside hydrolase family (GH) 39 were classified into the same subgroup with conserved amino acid residue positions participating in substrate recognition. Protein-ligand docking revealed that seven of these positions were probably essential to bind xylose-glucose, which is linked by a beta-1,2-glycosidic bond. Amino acid residues in five of the seven positions are invariant, while those in two of the seven positions are variable with low frequency. Both the wild-type beta-xylosidase rJB13GH39 and its mutants with mutation at the two positions exhibited beta-1,2-xylosidase activity, as they hydrolyzed o-nitrophenyl-beta-D-xylopyranoside and transformed notoginsenosides R-1 and R-2 to ginsenosides Rg(1) and Rh-1, respectively. The results suggest that all of these characterized GH 39 beta-xylosidases probably show beta-1,2-xylosidase activity, which should be assigned an EC number with these beta-xylosidases as representatives.
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
WOS记录号	WOS:000462260500017
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/67664]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Zhou, Junpei; Huang, Zunxi
作者单位	1.Yunnan Normal Univ, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ, Kunming 650500, Yunnan, Peoples R China 2.Yunnan Normal Univ, Coll Life Sci, 768 Juxian St, Kunming 650500, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rui,Li, Na,Xu, Shujing,et al. Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(11):3220-3228.
APA	Zhang, Rui.,Li, Na.,Xu, Shujing.,Han, Xiaowei.,Li, Chunyan.,...&Huang, Zunxi.(2019).Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(11),3220-3228.
MLA	Zhang, Rui,et al."Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.11(2019):3220-3228.