Mixture Toxicity of Bensulfuron-Methyl and Acetochlor to Red Swamp Crayfish (Procambarus clarkii): Behavioral, Morphological and Histological Effects

doi:10.3390/ijerph14121466

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 淡水生态学研究中心 > 期刊论文

	Mixture Toxicity of Bensulfuron-Methyl and Acetochlor to Red Swamp Crayfish (Procambarus clarkii): Behavioral, Morphological and Histological Effects
	Yu, Jixin 1,2; Xu, Elvis Genbo 3; Ren, Yan 1,2; Jin, Shiyu 1,2; Zhang, Tanglin 1; Liu, Jiashou 1; Li, Zhongjie 1
刊名	INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
	2017-12-01
卷号	14 期号:12 页码:15
关键词	Procambarus clarkii mixture of bensulfuron-methyl and acetochlor acute toxicity LC50 behavior histopathology rice herbicide
ISSN号	1660-4601
DOI	10.3390/ijerph14121466
英文摘要	The mixture of bensulfuron-methyl and acetochlor (MBA) has been widely applied as a rice herbicide in China, but the mixture toxicity of MBA to aquatic organisms is largely unknown. The current study aims to investigate the acute effects of MBA to juvenile red swamp crayfish, Procambarus clarkii. Firstly, a 96 h semi-static exposure was conducted to determine the Lethal Concentration 50 (LC50) values at 24, 48, 72 and 96 h, as well as to assess the behavioral and morphological effects. A second 96 h exposure was conducted at an MBA concentration of 50% of the 96 h LC50 (72.62 mg/L) to assess the histological changes in the gill, perigastric organ, muscle, heart, stomach, and midgut. The results showed that MBA exhibited low acute toxicity with the 24, 48, 72 and 96 h LC50 values of 191.25 (179.37-215.75), 166.81 (159.49-176.55), 154.30 (148.36-160.59) and 145.24 (138.94-151.27) mg/L, respectively. MBA-exposed crayfish showed body jerk, belly arch, equilibrium loss, body and appendage sway, and lethargy; and the dead crayfish showed dark gray or grayish-white body color and separated cephalothorax and abdomen. At 72.62 mg/L, MBA exposure caused significant histopathological alterations, mainly including the cuticular and epithelial degeneration of all the gills; atrophy of tubule lumina and cellular vacuolation of the perigastric organs (61.15 +/- 9.90% of the tubules showed lesions); epithelial hyperplasia (48.40 +/- 9.00%), myocardial fibers and epithelial cell lysis (17.30 +/- 2.01%), and hemocytic infiltration of the hearts; cuticular swelling (15.82 +/- 2.98%) and vacuolate connective tissue (11.30 +/- 2.47%) of the stomachs; atrophied bladder cell and fragmented longitudinal muscles (95.23 +/- 4.77%) of the midguts; and slight myofibers fragmentation and lysis (7.37 +/- 0.53%) of the abdominal muscles. Our results indicate that MBA can cause behavioral, morphological and histopathological effects on juvenile P. clarkii at relatively high concentrations, but its acute toxicity is low compared with many other common herbicides.
资助项目	Special Project of Science and Technology Department of Hubei Province[2016ABA123] ; Special Fund for Agro-scientific Research in the Public Interest[201203081] ; R&D Project of the Ministry of Science and Technology of China[2015BAD13B02]
WOS关键词	DEGRADATION-PRODUCTS ; HERBICIDE ACETOCHLOR ; TISSUE DISTRIBUTION ; RIVER-BASIN ; WATER ; INSECTICIDE ; PESTICIDES ; EXPOSURE ; JUVENILE ; FISH
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000423699400030
资助机构	Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Project of Science and Technology Department of Hubei Province ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; Special Fund for Agro-scientific Research in the Public Interest ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China ; R&D Project of the Ministry of Science and Technology of China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/30024]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者	Zhang, Tanglin
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA
推荐引用方式 GB/T 7714	Yu, Jixin,Xu, Elvis Genbo,Ren, Yan,et al. Mixture Toxicity of Bensulfuron-Methyl and Acetochlor to Red Swamp Crayfish (Procambarus clarkii): Behavioral, Morphological and Histological Effects[J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH,2017,14(12):15.
APA	Yu, Jixin.,Xu, Elvis Genbo.,Ren, Yan.,Jin, Shiyu.,Zhang, Tanglin.,...&Li, Zhongjie.(2017).Mixture Toxicity of Bensulfuron-Methyl and Acetochlor to Red Swamp Crayfish (Procambarus clarkii): Behavioral, Morphological and Histological Effects.INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH,14(12),15.
MLA	Yu, Jixin,et al."Mixture Toxicity of Bensulfuron-Methyl and Acetochlor to Red Swamp Crayfish (Procambarus clarkii): Behavioral, Morphological and Histological Effects".INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 14.12(2017):15.