High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii

doi:10.1016/j.algal.2018.101400

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	High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii
	Miao, Rongli 1,2; Ma, Xiaocui 1,2; Deng, Xuan 1; Huang, Kaiyao 1
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2019-03-01
卷号	38 页码:13
关键词	Chlamydomonas Lipid droplets (LDs) Reactive oxygen species (ROS) Glutathione peroxidase (GPX) Mutants
ISSN号	2211-9264
DOI	10.1016/j.algal.2018.101400
通讯作者	Huang, Kaiyao(huangky@ihb.ac.cn)
英文摘要	Many microalgae accumulate large amounts of lipid droplets (LDs) following N deprivation. The triacylglycerols (TAGs) in these LDs can be used as a feedstock to produce biodiesel. To investigate the molecular mechanism underlying LD formation, we used a percoll gradient-based method to enrich for mutants with defects in LD formation and generated an insertional mutagenesis library containing > 11,000 Chlamydomonas reinhardtii transformants. One of the mutants harbored a mutation in a gene encoding a glutathione peroxidase (GPX5) that catalyzes the formation of water from hydrogen peroxide (H2O2) or organic hydroperoxide. The gpx5 mutant had a 35% reduction in the number of LDs and a 50% reduction in TAG content compared with the parental strain CC4348 at 24 h of N deprivation. The full-length of GPX5 or truncate GPX5 without the putative signal peptide or the putative signal peptide of GPX5 fused YFP localized in the cytoplasm, but not in chloroplasts. Complementation with full-length GPX5 rescued the mutant phenotypes, and the expression of GPX5 in a complemented strain L27 was increased 2-3 fold after 24 h of N deprivation. Moreover, the gpx5 mutant showed increased sensitivity to the singlet oxygen (O-1(2)) stress generated by the photosensitizer Rose Bengal (RB). The ROS concentration of the gpx5 was about 1.5 times that of CC4348 during N deprivation. Artificial increasing of ROS concentration in cells abolished the formation of LDs in Chlamydomonas. These data suggest that a high level of ROS attenuates LD formation.
资助项目	National High-tech R&D Program (863 Program)[2014AA022001]
WOS关键词	PEROXIDASE HOMOLOGOUS GENE ; SINGLET OXYGEN ; NITROGEN DEPRIVATION ; LIPID-ACCUMULATION ; REVERSE GENETICS ; STRESS-RESPONSE ; MUTANT LIBRARY ; GPXH GENE ; EXPRESSION ; SYSTEMS
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000458402800018
资助机构	National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program)
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28616]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者	Huang, Kaiyao
作者单位	1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Miao, Rongli,Ma, Xiaocui,Deng, Xuan,et al. High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,38:13.
APA	Miao, Rongli,Ma, Xiaocui,Deng, Xuan,&Huang, Kaiyao.(2019).High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,38,13.
MLA	Miao, Rongli,et al."High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 38(2019):13.